Mouse IgG (H+L), Human ads Antibody : HRP
Referentie OASB01516
Formaat : 1.0mL
Merk : Aviva Systems Biology
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Telefoonnummer : +1 850 650 7790
Goat Anti-Mouse IgG(H+L) Antibody - HRP Conjugated (OASB01516)
| Datasheets/Manuals | Printable datasheet for Goat Anti-Mouse IgG(H+L) Antibody - HRP Conjugated (OASB01516) |
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| Tested Species Reactivity | Mouse |
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| Predicted Species Reactivity | Mouse |
| Clonality | Polyclonal |
| Isotype | IgG |
| Host | Goat |
| Conjugation | HRP |
| Application | ELISPOT, IHC-F, IHC-P, ICC, WB |
| Additional Information | Description: Pepsin digest of Goat Anti-Mouse IgG(H+L), Human ads - Pooled antisera from goats hyperimmunized with mouse IgG; purified by affinity chromatography on mouse IgG covalently linked to agarose; reacts with the heavy and light chains of mouse IgG1, IgG2a, IgG2b, IgG2c, and IgG3 and with the light chains of mouse IgM and IgA; cross-adsorbed against human immunoglobulins and pooled sera for minimal reactivity |
| Reconstitution and Storage | Store at 2-8C |
| Immunogen | Mouse IgG |
| Purification | Gel filtration chromatography of pepsin digested antibody. |
| Concentration | Lot specific |
| Specificity | IgG(H+L) |
| Characterization | To insure lot- to- lot consistency, each batch of product is tested by ELISA, FLISA, and/or flow cytometry forconformance with characteristics of a standard reference reagent. |
| Warning | - The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM boratebuffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C.- The fluorescein (FITC) and Cyanine 5 (CY5) conjugates are supplied as 0.5 mg in 1.0 mL PBS/NaN3.Store at 2- 8 C.- The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mMMgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2- 8 C or long- term at - 20C.- The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50%PBS, pH 7.4. No preservative added. Store at 2- 8 C or long- term at - 20C.- The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2- 8 C.- The R- phycoerythrin (R- PE) and allophycocyanin (APC) conjugates are supplied as 0.25 mg in 1.0 mL ofPBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze!- The Spectral Red (SPRD) and R- phycoerythrin- Cyanine 5.5 (R- PE- CY5.5) conjugates are suppliedas 0.25 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze!- Protect fluorochrome- conjugated forms from light. Reagents are stable for the period shown on the label ifstored as directed. |
| Dilution | Immunofluorescence: ELISA: FITC conjugate BIOT conjugate R-PE, APC, SPRD, CY5 and R-PE-CY5.5 conjugates AP conjugate HRP conjugate BIOT conjugate <= 1 ug/106 cells <= 1 ug/106 cells <= 0.1 ug/106 cells 1:2,000-1:4,000 1:4,000-1:8,000 1:5,000-1:20,000 |
| Application Info | Indirect immunofluorescent staining in conjunction with mouse monoclonal antibodies, Enzyme-Linked-Immunosorbent-Assay (ELISA), Fluorescent-Linked-Immunosorbent-Assay (FLISA), Western blotting, Dot-and slot-immunoblotting, Immunohistochemistry |
| Other Applications Data | Since applications vary, you should determine the optimum workingdilution of the product that is appropriate for your specific need. |
| Storage | - The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM boratebuffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C - The fluorescein (FITC) and Cyanine 5 (CY 5) conjugates are supplied as 0.5 mg in 1.0 mL PBS/NaN3 Store at 2- 8 C - The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mMMgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2- 8 C or long- term at - 20 C - The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50%PBS, pH 7.4. No preservative added. Store at 2- 8 C or long- term at - 20 C - The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2- 8 C - The R- phycoerythrin (R- PE) and allophycocyanin (APC) conjugates are supplied as 0.25 mg in 1.0 mL ofPBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze! - The Spectral Red (SPRD) and R- phycoerythrin- Cyanine 5.5 (R- PE- CY 5.5) conjugates are suppliedas 0.25 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze! - Protect fluorochrome- conjugated forms from light. Reagents are stable for the period shown on the label ifstored as directed. |
| Cross Absorption | Pooled human sera and purified human paraproteins. |
| Gene Full Name | Ig gamma-1 chain C region |
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