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> Mouse IgG1 antibody (Biotin)

Mouse IgG1 antibody (Biotin)

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Referentie orb347598-1mg

Formaat : 1mg

Merk : Biorbyt

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    Mouse IgG1 antibody (Biotin)

    Catalog Number: orb347598

    Catalog Numberorb347598
    CategoryAntibodies
    DescriptionMouse IgG1 antibody (Biotin)
    Species/HostRabbit
    ClonalityPolyclonal
    Tested applicationsELISA, IHC, WB
    ReactivityMouse
    IsotypeIgG
    ImmunogenMouse IgG1 heavy chain
    Concentration1.0 mg/mL
    Dilution rangeELISA: 1:250,000, IHC: 1:500 - 1:3,000, WB: 1:1,000 - 1:5,000
    Form/AppearanceLyophilized
    PurityMouse IgG1 antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum, Mouse IgG and Mouse Serum. Specificity was confirmed by ELISA. Typically, less than 1% cross-reactivity was observed against other Mouse heavy chain isotypes.
    ConjugationBiotin
    StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Buffer/Preservatives0.01% (w/v) Sodium Azide
    Alternative namesRabbit Anti-Mouse IgG1 (Gamma 1 chain) Antibody bi
    NoteFor research use only
    Application notesMouse IgG1 secondary antibody conjugated to Biotin is available in a variety of formats. Anti-Mouse IgG1 Biotin Antibody has been tested by ELISA and western blot and is suitable for ELISA, Immunohistochemistry western blotting as well as other anti IgG1 antibody based assays.
    Expiration Date12 months from date of receipt.
    Mouse IgG1 antibody (Biotin)

    ELISA Results of Rabbit Anti-Mouse IgG1 Antibody Biotin Conjugation tested against purified Mouse IgG1 Biotin. Each well was coated in duplicate with 1.0 µg of Mouse IgG1 (p/n orb343776). The working dilution is 1:146000. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer, Streptavidin-HRP conjugated (p/n orb348767) and TMB substrate (p/n orb348651).