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Mouse Monoclonal Anti-Blood Group Antigen A (CD173) [Clone HE-10]
Referentie NB-36-00249-P1
Formaat : 100ug
Merk : Neo Biotech
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Printable Datasheet
Blood Group Antigen A (CD173) Antibody [HE-10]
Catalog Number Formulation Size Price
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human Colorectal Carcinoma stained with Blood Group Antigen A Monoclonal Antibody (HE-10)
Applications & Dilutions
Applications Tested Dillution Protocol Note
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Summary
This MAb preferably reacts with determinants of chain A and H type 3(Gal1-3GalNAc-R) and 4 (Gal1-3GalNAc-R), but not with type 1 and 2 chain structures. It is not reactive with immuno-dominant A trisaccharide. This MAb is applicable for tissue staining in tumor patients with blood groups A and AB. It shows a highly heterogeneous reactivity in human colon tumor tissue and adjacent mucosa. Blood-group antigens are generally defined as molecules formed by sequential addition of saccharides to the carbohydrate side chains of lipids and proteins detected on erythrocytes and certain epithelial cells. The A, B and H antigens are reported to undergo modulation during malignant cellular transformation. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens.
Product Properties & Targets
Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunofluorescence, Immunohistochemistry
Species Reactivity
Human
Research Areas
Cardiovascular
Isotype / Light Chain
IgM / Kappa
Cellular Localization
Golgi apparatus, Golgi stack membrane, Secreted
Gene Name
ABO
Positive Control
KG1 cells. Human colorectal carcinoma tissues.
Immunogen
Mixture of erythrocytes of blood group Aand glyco protein fraction isolated from the saliva of secretors with blood group A
Alternate Names
Histo-blood group ABO system transferase, Fucosylglycoprotein 3-alpha-galactosyltransferase, Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase, Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase, Glycoprotein-fucosylgalactoside alpha-galactosyltransferase, Histo-blood group A transferase, Histo-blood group B transferase, NAGAT, A transferase; ABO; B transferase; CD173; Fucosylglycoprotein 3-alpha-galactosyltransferase; Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase; Glycoprotein-fucosylgalactoside alpha-galactosyltransferase; Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase; Histo-blood group A transferase; Histo-blood group B transferase; NAGAT
Functions
- This protein is the basis of the ABO blood group system. The histo-blood group ABO involves three carbohydrate antigens: A, B, and H. A, B, and AB individuals express a glycosyltransferase activity that converts the H antigen to the A antigen (by addition of UDP-GalNAc) or to the B antigen (by addition of UDP-Gal), whereas O individuals lack such activity.
Key References
- Nemec M et al. Murine monoclonal antibodies to human A erythrocytes: differential reactivity with N-acetyl-D-galactosamine. Vox Sang 52:125-8 (1987).
- J. Vaak et al. (1989) Detection of blood group A antigen expression in human colon cancer using monoclonal antibodies with different specificities. Neoplasma, 36: 479-487.
PubMed Links
- https://pubmed.ncbi.nlm.nih.gov/2104828/
- https://pubmed.ncbi.nlm.nih.gov/2333095/
- https://pubmed.ncbi.nlm.nih.gov/7598760/
- https://pubmed.ncbi.nlm.nih.gov/7779106/
- https://pubmed.ncbi.nlm.nih.gov/16533287/
- https://pubmed.ncbi.nlm.nih.gov/17764507/
- https://pubmed.ncbi.nlm.nih.gov/18513251/
- https://pubmed.ncbi.nlm.nih.gov/15489334/
- https://pubmed.ncbi.nlm.nih.gov/12829588/
- https://pubmed.ncbi.nlm.nih.gov/8839869/
- https://pubmed.ncbi.nlm.nih.gov/9800297/
- https://pubmed.ncbi.nlm.nih.gov/15104652/
- https://pubmed.ncbi.nlm.nih.gov/1449469/
- https://pubmed.ncbi.nlm.nih.gov/2121736/
- https://pubmed.ncbi.nlm.nih.gov/10462501/
- https://pubmed.ncbi.nlm.nih.gov/12198488/
- https://pubmed.ncbi.nlm.nih.gov/12529355/
- https://pubmed.ncbi.nlm.nih.gov/12972418/
- https://pubmed.ncbi.nlm.nih.gov/15475562/
- https://pubmed.ncbi.nlm.nih.gov/16326711/
- https://pubmed.ncbi.nlm.nih.gov/17259183/
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.
Supplied as
200ug/ml of Ab Purified from Bioreactor Concentrate. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
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