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> MPO, Mouse, ELISA kit

MPO, Mouse, ELISA kit

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Referentie HK210-01

Formaat : 1x96det.

Merk : Hycult Biotech

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MPO, Mouse, ELISA kit-0
MPO, Mouse, ELISA kit-502

MPO, Mouse, ELISA kit

The mouse MPO ELISA kit is to be used for the in vitro quantitative determination of mouse MPO in plasma, tissue homogenate and cell culture supernatant samples.

Enhance Your Research with the MPO Mouse ELISA Kit

Experience unparalleled accuracy in your biological assays with the MPO Mouse ELISA Kit, meticulously designed for the in vitro quantitative determination of mouse Myeloperoxidase (MPO) in plasma, tissue homogenates, and cell culture supernatant samples. Our kit provides a robust solution for researchers focusing on myeloid lineage cells, where MPO, a critical alpha2beta2 heteromultimer glycoprotein, plays a pivotal role.

The MPO Mouse ELISA Kit illuminates the abundant presence of MPO in the azurophilic granules of polymorphonuclear neutrophils, highlighting its essential function during the phagocytic lysis of foreign particles. This process is vital for organism defense, primarily through the production of hypochlorous acid (HOCl), a potent oxidant. Rapidly released by activated polymorphonuclear neutrophils, MPO’s role extends far beyond a simple cell component.

Dive deeper into the pathophysiological significance of MPO, a key player implicated in a myriad of diseases such as atherosclerosis, lung cancer, Alzheimer’s disease, and multiple sclerosis. Its connection with autoimmune responses, particularly in Wegener’s disease through antibodies targeting MPO, underscores its clinical relevance. MPO deficiency, once deemed rare, is not gaining more attention.

Trust in our kit to advance your studies that pave the way for groundbreaking discoveries.

Perform curve fitting with Assayfit Pro with the .

Application
The mouse MPO ELISA kit is to be used for the in vitro quantitative determination of mouse MPO in plasma, tissue homogenate and cell culture supernatant samples. Note that most reliable results are obtained with heparin plasma.

Principle
The mouse MPO ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 3½ hours. The efficient format of a plate with twelve disposable 8-well strips allows free choice of batch size for the assay. Samples and standards are incubated in microtiter wells coated with antibodies recognizing mouse MPO. Biotinylated tracer antibody will bind to captured mouse MPO. Streptavidin-peroxidase conjugate will bind to the biotinylated tracer antibody. Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB). The enzyme reaction is stopped by the addition of oxalic acid. The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the mouse MPO standards (log). The mouse MPO concentration of samples, which are run concurrently with the standards, can be determined from the standard curve.

Linearity
The linearity of the assay was determined by serially diluting a serum sample containing 49.62 ng/ml mouse MPO. The diluted samples were measured in the assay. The line obtained a slope of 1.03 and a correlation coefficient of 0.998.

Product type
Assays
Amount
1 x 96 det., 2 x 96 det.
Standard range
1.6-100 ng/ml
Detection level
1.6 ng/ml
Working volume
100 µl/well
Species
Mouse
Cross reactivity
Rat – Weak
Storage and stability
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least six months.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Disease
Cardiology and metabolism, Pulmonology
Safety Data Sheet
SDS - HK210
63 kb
Assay Manual
HK210
365 kb

Calculate your ELISA data easily

With the ELISA calculator you can easily calculate ELISA data. Assayfit Pro helps to perform curve fitting. The calculator generates advanced reports, fit graph, fit parameters and goodness of fit are shown.

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