Narcissus pseudonarcissus (NPL/NPA)

Narcissus pseudonarcissus lectin or agglutinin (NPL/NPA) is affinity purified composed of four subunits. It agglutinates rabbit erythrocytes but is non-reactive with human erythrocytes. NPA has an isoelectric point between pH 4.2 and pH 4.6 with a specific binding activity toward alpha-D-mannose, although studies have suggested that the purified lectin may have greater affinity for internal mannosyl residues than for nonreducing terminal units. A buffer washed column elutes the sugar alpha-methylmannoside. Human studies have found NPA to be nearly non-mitogenic, but it has been reported through an in vitro study that mitogenicity of NPA is age-related, with lymphoproliferative response of peripheral blood mononuclear cells (PBMC) in umbilical cord blood has a sevenfold increase than adult blood. NPA has also been found to be mitogenic for PBMC from lambs and not adult sheep.||Source:|Lent lily||Blood Specificity:|Rabbit Agglutinin||Sugar Specificity:|alpha-Mannose||Inhibitory Carbohydrate:|alpha-Methylmannoside||Divalent Ions:|None Required||Storage and Stability:|Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.