NCI-H146
Referentie 300182
Formaat : 1cryovial
Merk : CLS Cell Lines Service
NCI-H146 Cells
General information
Description | The NCI-H146 cell line was derived by A.F. Gazdar and associates in 1979 from the pleural fluid of a patient with small cell cancer of the lung. The bone marrow specimen was taken prior to therapy. |
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Organism | Human |
Tissue | Lung |
Disease | Small cell carcinoma |
Metastatic site | Bone marrow |
Synonyms | H146, H-146, NCIH146 |
Characteristics
Age | 59 years |
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Gender | Male |
Ethnicity | Caucasian |
Morphology | Epithelial-like |
Growth properties | Aggregates in suspension |
Identifiers / Biosafety / Citation
Citation | NCI-H146 (Cytion catalog number 300182) |
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Biosafety level | 1 |
Expression / Mutation
Receptors expressed | insulin-like growth factor II receptor (IGF II) |
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Protein expression | The cells stain positively for vimentin and keratin, but are negative for neurofilament triplet protein. |
Antigen expression | The line expresses elevated levels of four biochemical markers: neuron specific enolase, brain isoenzyme of creatine kinase, L-DOPA decarboxylase and bombesin-like immunoreactivity |
Isoenzymes | G6PD, B, PGM1, 1-2, PGM3, 1-2, ES-D, 1, Me-2, 2, AK-1, 1, GLO-1, 1, Phenotype Frequency Product = 0.0009 |
Tumorigenic | Forms transplantable tumors in nude mice which histologically resemble tumor cells from the original biopsy specimen |
Products | The cells produce relatively high amounts of c-myc mRNA, but c-myc DNA sequences are not amplified. The cells do not express vasopressin, oxytocin or gastrin releasing peptide. |
Ploidy status | Aneuploid |
Karyotype | This is a near triploid human cell line. The modal chromosome number is 68, but cells with 66, 70 and 71 chromosomes also occurred frequently. The x chromosomes were paired, and no Y chromosome was detected in QM stained preparations. |
Handling
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
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Medium supplements | Supplement the medium with 10% FBS |
Subculturing | The cells should be subcultured by transferring part of the suspension into fresh new cell culture flasks prefilled with fresh medium. Alternatively, the clusters may be collected by centrifugation and resuspended in fresh medium. |
Split ratio | A ratio of 1:2 to 1:6 is recommended |
Seeding density | 1 to 2 x 10^5 cells/ml |
Fluid renewal | 2 to 3 times per week |
Freezing recovery | After thawing allow the cells to recover from the freezing process for at least 24 to 48 hours. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile | Amelogenin: x,x |
HLA alleles | A*: 01:01:01, 03:01:01 B*: 14:02:01, 44:03:01 C*: 08:02:01, 16:01:01 DRB1*: 08:01:01, 15:01:01G DQA1*: 01:02:01, 04:01:01 DQB1*: 04:02:01, 06:02:01 DPB1*: 02:01:02, 05:01:01 E: 01:01:01 |