Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2/COVID-19) Trimeric Spike 1 Protein Antibody is a Mouse Monoclonal Antibody against SARS-CoV-2/COVID-19 Trimeric S1 Protein. The SARS-CoV-2 Spike Protein (S protein) is a viral protein that allows the entry of SARS-CoV-2 into human cells. The protein forms trimers on the viral capsid and binds to human Angiotensin Converting Enzyme 2 (ACE2) located on the cell surface. The protein has a cleavage site between the S1 and S2 subunits that is targeted by the human enzyme Furin, and it may also cause the development of a syncytium (cell fusion). Antibodies to S protein can prevent viral entry as well as target the virus for further immune action. This antibody is designed to work with saliva patient samples that do not require lysis. It is recommended to use abx024240 as the capture antibody and abx024241 (this antibody) as the detection antibody. In an ELISA with TMB substrate, this pair can detect SARS-CoV-2 trimeric spike protein with a sensitivity of approximately 300 pg/ml.
Research Articles on Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Trimeric Spike 1 Protein
| Target | Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Trimeric Spike 1 Protein |
| Clonality | Monoclonal |
| Clone | G652 |
| Reactivity | Virus |
| Tested Applications | ELISA, WB, LF |
| Host | Mouse |
| Recommended dilutions | Optimal dilutions/concentrations should be determined by the end user. |
| Conjugation | Unconjugated |
| Immunogen | Recombinant SARS-CoV-2 S1 Protein |
| Epitope Region | Recognizes a linear epitope that is not conformation-dependent. |
| Isotype | IgG2b |
| Form | Liquid |
| Purity | > 90% |
| Purification | Purified by Protein A affinity chromatography. |
| Storage | Store at 2°C to 8°C. |
| Buffer | PBS, pH 7.4. Does not contain preservatives. |
| Specificity | Binds to recombinant SARS-CoV-2 trimeric spike protein. Recognizes the membrane bound form from the cell line expressing full-length SARS-CoV-2 protein. This antibody is specific to SARS-CoV-2 Spike trimeric protein and does not cross-react with recombinant SARS-CoV, HCoV-229E, HCoV-HKU1, HCoV-NL63 and HCoV-OC43. |
| Concentration | 1.15 mg/ml |
| Availability | Shipped within 10-20 working days. |
| Note | This product is for research use only. |
| Directions for use | Centrifuge before opening to ensure complete recovery of vial contents. |