Phosphofructokinase(PFK)Activity Assay Kit

Referentie E-BC-K612-M-96T

Formaat : 96T

Merk : Elabscience

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Detection Principle Phosphofructokinase (also known as 6-phosphofructokinase; PFK) is a class of kinases that act on fructose-6-phosphate. Phosphofructokinase catalyzes fructose-6-phosphate and ATP to produce fructose-1, 6-diphosphate and ADP, and pyruvate kinase and lactate dehydrogenase further catalyze the oxidation of NADH to NAD+. The activity of PFK can be reflected by the determination of NADH decline rate at 340 nm.
Synonyms PFK
Sample Type Serum,Plasma,Tissue,Cell sample
Detection Method Colorimetric method
Detection Instrument Microplate reader (330-350 nm, optimum wavelength: 340 nm)
Research Area Glycolysis And Lipid Metabolism ,  Energy Metabolism
Other Reagents Required Normal saline (0.9% NaCl)
Storage This product can be stored at -20°C for 12 months with shading light.
Valid Period 12 months
Sensitivity 0.27 U/L
Detection Range 0.27-32.29 U/L
Precision inter-assay CV: 0.08 | intra-assay CV: 0.03
Assay Time 30 min
Cat.No. Product Name Sizes
E-BC-K011-M Glucose-6-phosphate (G6P) Colorimetric Assay Kit 96T
E-BC-K056-M Glucose-6-Phosphate Dehydrogenase (G-6-PD) Activity Assay Kit 96T
E-BC-K234-M Glucose (Glu) Colorimetric Assay Kit (GOD-POD Method) 500Assays , 96T , 48T

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type Dilution Factor
10% Rat liver tissue homogenate 1
10% Rat kidney tissue homogenate 1
10% Rat brain tissue homogenate 1
10% Mouse liver tissue homogenate 1
10% Mouse spleen tissue homogenate 1
10% Mouse kidney tissue homogenate 1
10% Mouse heart tissue homogenate 1
Rat serum 1
Rat plasma 1
Jurkat cell 1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.

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