Rabbit polyclonal antibody to USF1
Referentie CQA2308-50ul
Formaat : 50ul
Merk : Cohesion Biosciences
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Telefoonnummer : +1 850 650 7790
- Western blot analysis of USF1 expression in Hela (A), HEK293T (B) whole cell lysates.
- Immunohistochemical analysis of USF1 staining in mouse lung formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
- Immunofluorescent analysis of USF1 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
| Product Name | Anti-USF1 Antibody |
| Cat No: | CQA2308 |
| Source: | Rabbit |
| Reactivity: | H, M, R |
| Applications: | WB, IH, IF/IC |
| *Application Key: | |
| E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference | |
| *Species Reactivity Key: | |
| H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish | |
Description: Rabbit polyclonal antibody to USF1 Immunogen: Recombinant full length protein of human USF1 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200) Gene Symbol: USF1 Alternative Names: BHLHB11; USF; Upstream stimulatory factor 1; Class B basic helix-loop-helix protein 11; bHLHb11; Major late transcription factor 1Entrez Gene (Human): Entrez Gene (Mouse) : SwissProt (Human): SwissProt (Mouse) : Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.