The Substance P (SP) BioAssay™ ELISA Kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of SP in bovine serum, plasma, tissue homogenates, cell lystates, cell culture supernates and other biological fluids.||Intended Use:|BioAssay™ ELISA Kit for Substance P (SP) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids||Detection Range:|12.35-1000pg/ml||Sensitivity:|5.52pg/ml||Intra-Assay: |CV<10%||Inter-Assay: |CV<12%||Kit Components:|*366584A: Microtiter Strips, 1x96 wells, Pre-coated, ready to use|*366584B: Standard, 2x1 vial|366584C: Standard Diluent, 1x20ml|*366584D: Detection Reagent A (green), 1x120ul|*366584E: Detection Reagent B (red), 1x120ul|366584F: Assay Diluent A, 1x12ml|366584G: Assay Diluent B, 1x12ml|366584H: TMB Substrate, 1x9ml|*366584J: Positive Control, 1x1vial, xxpg/ml, (Lyophilized)|366584K: Stop Solution, 1x6ml|366584L: Wash Buffer, 30X, 1x20ml||Precaution:|The Stop Solution (366584K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.||Storage and Stability:|Store *366584A, *366584B, *366584D, *366584E and *366584J at -20°C. After reconstitution of *366584J, store at 4°C and use within 5 days. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.||Assay Procedure Summary:|1. Prepare all reagents, samples and standards.|2. Add 50ul standard or sample to each well.|3. Add 50ul prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C.|4. Aspirate and wash 3 times.|5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C.|6. Aspirate and wash 5 times.|7. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37°C.|8. Add 50ul Stop Solution. Read at 450nm immediately.