Tissue, Membrane Protein, Human Tumor Cell Line, A431 (Human Epidermoid Carcinoma) HisTek™
Referentie T5595-2417-100ug
Formaat : 100ug
Merk : US Biological
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T5595-2417 Tissue, Membrane Protein, Human Tumor Cell Line, A431 (Human Epidermoid Carcinoma) HisTek™
Clone Type
PolyclonalGrade
Molecular Biology GradeShipping Temp
Dry IceStorage Temp
-70°CTissue Protein Lysate produced under consistent processing conditions to ensure intact structure and original nature. Total protein is prepared from whole-tissue homogenates and presents a consistent pattern on SDS-PAGE analysis. ||Applications:|• Electrophoresis, Western Blotting and Immunoprecipitation|• Enzymatic Activity|• Protein-DNA Interaction|• Protein-Protein Interaction|• Tissue-Specific Expression Identification||Additional Data Supplied: All tissue protein samples are supplied with verified donor age and sex.
Applications
Preparation:|Total protein is prepared from whole-tissue homogenates, immediately frozen in liquid nitrogen after excision, and then stored at -70°C.||Quality Control:|1. The isolated protein pattern on SDS-PAGE gel is visualized by Coomassie blue staining. The pattern is consistent with each lot.|2. The isolated protein is Western analyzed by either GAPDH or beta-actin antibody, and the expression level is consistent with each lot.||Storage and Stability:|For extended storage, keep frozen at -80°C in working aliquots. For continuous use, stored at 2-8°C. Repeated freezing and thawing is not recommended.||Important Note: This product is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Form
Supplied as a frozen liquid in HEPES, pH 7.9, MgCl2, KCl, EDTA, sucrose, glycerol, sodium deoxycholate, NP-40 and a cocktail of protease inhibitors.