TNFa, Recombinant, Mouse, aa80-235 (Tumor Necrosis Factor, DIF, TNF-alpha, TNFalpha, Tnfsf1a, TNFSF2, Cachectin)
Referentie 377989-20ug
Formaat : 20ug
Merk : US Biological
377989 TNFa, Recombinant, Mouse, aa80-235 (Tumor Necrosis Factor, DIF, TNF-alpha, TNFalpha, Tnfsf1a, TNFSF2, Cachectin)
Clone Type
PolyclonalGrade
Highly PurifiedAccession #
NP_038721Shipping Temp
Blue IceStorage Temp
-20°CTNFa, also known as tumor necrosis factor, binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia. ||Source:|Recombinant protein corresponding to aa80-235 from mouse TNFa, was expressed in E. coli.||Molecular Weight: |~17.4kD (157aa), confirmed by MALDI-TOF||Endotoxin: |<1EU/1ug (determined by LAL method)||Biological Activity:|Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is ≤0.06ng/ml.||Amino Acid Sequence:|MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL||Storage and Stability:|May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.