AP151-01 | TransStart® TopTaq DNA Polymerase Clinisciences

Trans109 Chemically Competent Cell

Catalog Number： CD301-02

Price：Please inquire first

Specification：

10×100 µl

20×100 µl

Manual MSDS

Normal version download HD version download

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Product Details

Trans109 Chemically Competent Cell is specifically designed for chemical transformation of DNA. It permits a transformation efficiency of over 10⁸ cfu/μg DNA (tested by pUC19 plasmid DNA).

Genotype
endA1 recA1 gyrA96 thi-1 hsdR17 (rk^-, mk⁺) relA1 supE44 D (lac-proAB) [F′traD36 proAB la^qIqZΔM15]

Features
• High transformation efficiency: >10⁸ cfu/μg ( pUC19 DNA).
• The lowest homologous recombination favorable for plasmid DNA preparation.
• Routine cloning.
• Blue/white selection.

Storage
at -70°C for six months. Do not store in liquid nitrogen

Shipping
Dry ice (-70℃)

References

Li BJ, Wang H, Gong T, et al. Improving 10-deacetylbaccatin III-10-b-Oacetyltransferase catalytic fitness for Taxol production[J]. Nature Communications,2017, 18(8):15544.
Guo Z , Kang S , Zhu X , et al. Down-regulation of a novel ABC transporter gene (Pxwhite) is associated with Cry1Ac resistance in the diamondback moth, Plutella xylostella (L.)[J]. Insect Biochemistry and Molecular Biology, 2015, 59:30-40.
Zhang M , Jiang, Shaoâtong, Zheng Z , et al. Cloning, expression, and characterization of a novel xylose reductase from Rhizopus oryzae[J]. Journal of Basic Microbiology, 2015, 55(7):907-21.

FAQ

Cloning FAQs

TransStart® TopTaq DNA Polymerase

Referentie AP151-01

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