P1080-10ug | VEGFR-2, human recombinant protein Clinisciences

Vascular endothelial growth factor receptor 2 (VEGFR-2) belongs to the family of receptor tyrosine kinases (RTKs) and is almost exclusively restricted to endothelial cells. VEGFR-2 has a lower affinity for VEGF than the Flt-1 receptor, but a higher signaling activity. VEGFR-2 has seven immunoglobulin-like extracellular domains, a single transmembrane region and an intracellular split tyrosine kinase domain. VEGFR-2 mediates mitogenic activity in endothelial cells, which leading to their proliferation. The binding of VEGF165 to VEGFR-2 is dependent on heparin.

Recombinant human VEGFR-2 is produced in baculovirus as a monomeric, glycosylated, polypeptide and having a total molecular mass of 116 kDa. The soluble receptor protein contains only the first 7 extracellular domains, which contain all the information necessary for ligand binding. The VEGFR-2 is purified by proprietary chromatographic techniques.

Determined by its ability to abolish the binding of iodinated VEGF to solid surfaces or cell surfaces receptors.
ED₅₀	10.0 ng/ml.
Specific activity	100,000IU/mg.

Purity: ≥95%, by SDS-PAGE.
Endotoxin Level:<0.1 EU/μg

Gene ID:	3791	Accession #:	P35968
Alternate Names:	VEGFR-2, Fetal liver kinase 1, Kinase insert domain receptor, Protein-tyrosine kinase receptor flk-1.
Source:	Insect cells.	M.Wt:	116 kDa
Appearance:	Lyophilized protein	Storage:	Store at -20℃
Formulation:	Lyophilized from a sterile solution containing 25 mM MES pH 5.5 and 100 mM NaCl.
Solubility:	Reconstitute in sterile water to a concentration not less than 0.1 mg/ml. This solution can then be stored at 4℃ for 2-7 days. For long term storage it is recommended to add a carrier protein (0.1 % HSA or BSA) then store at -20℃. Avoid freeze-thaw cycles.
Shipping Condition:	Blue ice.	Handling:	Centrifuge the vial prior to opening.
Usage:	For Research Use Only! Not to be used in humans.

VEGFR-2, human recombinant protein

Referentie P1080-10ug

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