Vimentin (VIM) (HRP)

Referentie 172159-AF-HRP-100ul

Formaat : 100ul

Merk : US Biological

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172159-AF-HRP Vimentin (VIM) (HRP)

Clone Type
Polyclonal
Host
mouse
Source
human
Swiss Prot
P08670 (Human)
Isotype
IgG1
Grade
Affinity Purified
Applications
E IHC IP WB
Crossreactivity
Bo Ca Ch Fe Gt Hu Po
Shipping Temp
Blue Ice
Storage Temp
-20°C

This MAb reacts with a 58kD protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP’s) such as desmin, keratin, neurofilament, and glial fibrillary acid protein. Anti-vimentin alone is of limited value as a diagnostic tool; however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.||Applications: |Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested.||Recommended Dilution:|Western Blot: 0.5-1ug/ml|Immunoprecipitation: 1-2ug/500ug protein lysate|Immunohistochemistry (Frozen & Formalin-fixed): 0.5-1ug/ml for 30 minutes at RT|(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)|Optimal dilution for a specific application should be determined.||Positive Control:|Jurkat cells, Sarcomas, Melanomas||Storage and Stability:|Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. |For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.||Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG1|Clone No: SPM576|Host: mouse|Source: human|Concentration: As Reported |Form: Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with horseradish peroxidase (HRP).|Purity: Purified by Protein A/G affinity chromatography.|Immunogen: Human vimentin recombinant protein. Cellular Localization: Cytoplasmic|Specificity: Recognizes Vimentin. Species Crossreactivity: Human, bovine, Canine, Feline, Porcine, Goat and Chicken. Does not react with mouse and rat. ||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Human vimentin recombinant protein. Cellular Localization: Cytoplasmic
Form
Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with horseradish peroxidase (HRP).
Purity
Purified by Protein A/G affinity chromatography.
Specificity
Recognizes Vimentin. Species Crossreactivity: Human, bovine, Canine, Feline, Porcine, Goat and Chicken. Does not react with mouse and rat.
References
1. Osborn M et. al. European Journal of Cell Biology. 1984; 34:137-143.