4ABio

4A is een biotechbedrijf dat zich richt op activiteiten met betrekking tot antilichamen. 4A Biotech heeft een reeks ELISA-kits en honderden monoklonale antilichamen tegen kanker (met name leukemie) ontwikkeld, evenals producten voor immunologisch onderzoek.

Website: www.4ABio.com

 
Coating buffers

Coating buffers

Coating buffers are buffers for adsorptive immobilization of proteins and antibodies on plastic surfaces (for example microtiter plates) or other protein binding surfaces.

Depending on the protein or the antibody the pH of the Coating Buffer is crucial for efficient immobilization. Select from Coating Buffer pH 7.4 and pH 9.6, as the pH-value can have an influence on the steric structure of proteins or antibodies, thus affecting their immobilization.

For the optimization of the coating procedure of a newly developed immunoasay we strongly recommend testing the two Coating Buffers side by side. Depending on the surface as well as on proteins or antibodies the required incubation time can vary.
Consequently, individual optimization of the incubation procedure is recommended.

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SFQ012
 100ml