Reagentia en instrumenten voor immunologie, celbiologie en moleculaire biologie.
 

PhytoTechnology Labs


PhytoTechnology Laboratories focusses on providing products for plant tissue culture research and plant production. PhytoTech's products are focused on plant tissue culture but we also carry lines of products for plant molecular biology, phycologoy and seed testing applications. PhytoTechnology Laboratories is an industry leader in the manufacture of plant tissue culture media and we carry more than 150 stock formulations of media, and can produce custom formulations of your tissue culture media formulations. 

Discover some products :

  • Media optimization kit helps you develop an optimal media formulation for your crops. 
  • A new line of pre-poured, ready-to-use plant media in culture tubes, called PhytoReady. 
  • Two excellent new plastic culture vessels: the PTL-100 and the PT-12 Culture Jar. 


 
> PhytoTechnology Labs > Producten voor onderzoek > Moleculaire Biologie Gereedschap > Nucleic acids extraction > RNA extraction > Specific RNA type > Extraction and purification of total RNA > Extraction and purification of total RNA - Animal > Total RNA extraction - Precipitation
Total RNA extraction - Precipitation

Total RNA extraction - Precipitation


The most common organic extraction is the method using phenol-chloroform solution
Phenol extraction is a commonly used method for removing proteins from a DNA sample, e.g. to remove proteins from cell lysate during genomic DNA preparation.
Organic extraction involves the addition of and incubation in multiple different chemical solutions; including a lysis step, a phenol chloroform extraction, an ethanol precipitation, and washing steps.
Samples are homogenized in a phenol-containing solution and then centrifuged after chloroform addition. During centrifugation, the sample separates into three phases: an upper aqueous phase which contains RNA, an interface containing DNA, and a lower organic phase containing proteins and lipids.
The advantage of the phenol-chloroform method is the simplicity of the protocol, the rapid denaturation of nucleases and the stabilization of nucleic acids. The main disadvantage is the use of toxic chemicals phenol and chloroform, and there is an increased risk of contamination due to transferring the DNA between multiple tubes.

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