IHC buffers - Antigen retrieval

IHC buffers - Antigen retrieval


Most formalin-fixed tissues require an antigen retrieval step before immunohistochemical staining. Although fixation is essential for the preservation of tissue morphology, this process can also have a negative impact on IHC/ICC detection. Fixation can alter protein biochemistry such that the epitope of interest is masked and can no longer bind to the primary antibody. Methylene bridges formed during fixation cross-link proteins and mask antigenic sites. Antigen retrieval methods break these methylene bridges and expose antigenic sites, allowing antibodies to bind. The two methods for antigen retrieval are heat induced epitope retrieval (HIER) and enzymatic retrieval.

We offer a range of HIER Retrieval Buffers with different pH and packaging (ready-to-use or concentrated) to find the one that best suits your protocol.


Resultaat van uw zoeken : 335 product gevonden

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RUOCE / IVD
  • Unconjugated 262
  • human 6
  • guinea pig 4
  • mouse 4
  • rabbit 4
  • rat 4
  • goat 4
  • Buffers and reagents 240
  • Other products 59
  • Biochemicals 16
  • kit 6
  • Primary antibody 4
  • Secondary antibody 4
  • Protein/peptide 3
  • cell culture media 3
  • IHC 100
  • IF 18
  • Cell culture 3
  • Not Applicable 6
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Cond.
Price Bef. VAT
NB-23-00178-4
 50mL 
NB-23-00178-3
 125mL 
NB-23-00174
 100mL 
NB-23-00178-1
 1000mL 
NB-23-00172
 1000mL 
NB-23-00178-2
 1000mL 
NB-47-02328-250ML
 250ml 
NB-23-00173
 100mL 
NB-23-00175
 100mL 
NB-23-00176
 100mL 
NB-23-00177
 1000mL 
CBB999
 1000ml 
K038
 500ml 
GTX30709
 100ml