Propidium Iodide

Propidium Iodide

Propidium Iodide (PI) is a nucleic acid stain commonly used to identify dead cells in a population or as a counterstain in multicolor fluorescent techniques. It is a dye impermeable to the cell membrane that intercalates with nucleic acids, with an excitation maximum at 535 nm and an emission maximum at 617 nm. Propidium Iodide is excluded from viable cells with intact membranes, but it can easily penetrate dead or damaged cells, making it a useful tool for identifying cell viability in a population or as a counterstain in multicolor fluorescent techniques.

PI is used in a variety of applications, including DNA and RNA gel electrophoresis, flow cytometry, multiplexing, microscopy, direct labeling, hybridization, PCR, FISH, and probes conjugated to DIG-dUTP. It is also used in the assessment of apoptosis in certain cell populations in heterogeneous tissues, including bone marrow, lymph nodes, and the thymus.

Propidium Iodide is a highly sensitive and versatile nucleic acid stain commonly used to identify cell viability, assess apoptosis, and quantify DNA content in biological samples. 

PI intercalates with nucleic acids with a stoichiometric ratio of one dye per 4 to 5 base pairs with little sequence preference and is then detected by fluorescence microscopy and flow cytometry. The fluorescence of PI is increased 20 to 30 times when bound to nucleic acids, making it a highly sensitive stain for detecting dead cells.

Resultaat van uw zoeken : 126 product gevonden

Verfijn uw zoekopdracht :

RUOCE / IVD
  • Unconjugated 48
  • Biochemicals 60
  • Buffers and reagents 38
  • Inhibitor/Antagonist/Agonist 11
  • kit 8
  • Microspheres/Particles 3
  • Protein/peptide 3
  • Primary antibody 2
  • Other products 1
  • FC 16
  • ICC 2
  • IF 2
  • ELISA 1
  • Microscopy 1
  • Polyclonal 2
BRENG DE FILTERS AAN
INITIALISEREN


Referentie
Beschrijving
Cond.
Price Bef. VAT
NB-42-01948-100mg
 100mg 
NB-42-01948-250mg
 250mg 
40016
 100MG 
orb1215640-200Tests
 200Tests 
orb1225502-25mg
 25mg 
orb1215640-500Tests
 500Tests 
orb1225502-10mg
 10mg 
orb1306410-25mg
 25mg 
orb1306410-10mg
 10mg 
orb1306410-100mg
 100mg 
orb1306410-50mg
 50mg 
B7758-10mg
 10mg 
orb1671176-10mg
 10mg 
orb1671176-50mg
 50mg 
orb511131-1ml
 1ml 
10044-01
 100tests 
T2130-100mg
 100mg 
T2130-10mg
 10mg