NAD kinase activity assay
The NAD+ kinase activity assay is a pivotal biochemical technique used to measure the activity of NAD+ kinase, an enzyme responsible for phosphorylating NAD+ to produce NADP+. This conversion is crucial for maintaining the balance of the NAD/NADP+ pool, which plays a significant role in various metabolic processes, including cellular respiration, lipid synthesis, and the regulation of oxidative stress.
The assay typically involves incubating a reaction mixture containing NAD+, ATP, and the enzyme source under controlled conditions. Following incubation, the reaction is terminated, and the resulting NADP+ can be quantified using spectrophotometric methods or high-performance liquid chromatography (HPLC). One common approach to this assay is to couple the reaction with glucose-6-phosphate dehydrogenase (G6PDH), where the production of NADP+ leads to the reduction of NAD+ to NADH.
This assay is particularly valuable in research focused on metabolic regulation, cancer biology, and studying the effects of various compounds on cellular metabolism. Additionally, understanding NAD+ kinase activity has implications for therapeutic strategies targeting metabolic disorders and age-related diseases, making it an essential tool in both basic and applied research.
Resultaat van uw zoeken : 22 product gevonden
Verfijn uw zoekopdracht :
- human 6
- human 5
- all species 2
- kit 16
- ELISA/ELISPOT 6
- FA 6
- Enzyme-linked immunosorbent assay for Antigen Detection. 5
- ELISA 1