Long-term culture and high-resolution microscopy of spheroids, organoids and cells suspended on a non-adherent surface

• Superior to standard ultra-low attachment (ULA) surfaces: stable, biologically inert surface for long-term experiments without any cellular or biomolecular adhesion
• Excellent microscopic quality without any autofluorescence: 100% surface passivation combined with the highest optical quality of the ibidi polymer coverslip

Applications

• Generation and long-term culture of spheroids and organoids without scaffolds
• High resolution fluorescence microscopy of organoids, spheroids, embryoid bodies (EBs) and suspension cells
• Background-free analysis of cell-cell interactions
• Prevention of stem cell differentiation due to attachment
• Cell suspension culture in a permanently unattached state
• Assays for the formation of self-assembling tumor spheroids
• 3D models of tumor spheroids