µ-Slide I Luer 3D

A µ-Slide with one channel and three wells for culturing cells on a 3D gel matrix with defined flow

Create a perfusable cell monolayer on a gel matrix for various flow and shear stress applications
Establish and image endothelial barriers under in vivo-like conditions—no artificial filters or membranes
Easy handling: samples are placed into the open wells; perfusion is applied after closing the wells

Applications

Culture of adherent cells on a 3D gel matrix under flow conditions
Transendothelial migration studies under flow conditions
Establishment and microscopic read-out of endothelial barrier assays (without any artificial membrane)
Simulation of blood vessels with endothelial cell culture on a soft substrate
Long-term or short-term cell culture perfusion experiments with defined shear stress and optimal nutrient supply into 3D structures
Apical-basal cell polarity assays
Cell barrier model assays with apical-basal gradients
Cell boundary assays without filters or porous membranes
Rolling and adhesion assays using leukocytes
Inflammation studies
Transport studies

Technical Features

Can create low or high shear stress in the 0.6 mm channel
Initial open well format allows for easy sample preparation, then is simply closed with a coverslip for the application of flow
The well design corrects meniscus formation and allows for superb cell visualization in one focal plane
Can be used with all ECM hydrogels
(e.g., Collagen Type I, Matrigel, Fibrinogen)
Contains Luer adapters for easy tube and pump connection (e.g., to the ibidi Pump System)
ibidi Polymer Coverslip on top and bottom for high-resolution light microscopy
Partially compatible with upright microscopy
Defined shear stress levels

The Principle of the µ-Slide I Luer 3D

The μ-Slide I Luer 3D has three wells with one channel on top for culturing cells on a 3D gel matrix with defined flow. Each well can be filled with a gel, on which cells can be cultivated and microscopically investigated. The channel can be connected to a pump (e.g., to the ibidi Pump System) for the application of defined shear stress.

Using this method, an in vivo-like functional monolayer (e.g., an endothelial barrier) can be established on the gel matrix without any artificial filters or membranes involved. The μ-Slide I Luer 3D is especially designed for the simulation of blood vessels using endothelial cells.

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