Applied StemCell

Applied StemCell Inc. (ASC) is een snelgroeiend biotechnologiebedrijf waarvan het hoofdkantoor is gevestigd in Menlo Park, Californië.

Met solide expertise en uitgebreide ervaring in stamcel- en transgene technologieën, richt ASC zich op de toepassing van nieuwe technologieën voor stamcel- en transgene gemeenschappen.

Gebaseerd op eigen technologieën, richt Applied StemCell zich op de ontwikkeling en commercialisatie van:

  • Stamcelcultuur producten
  • Stamcellijnen en iPS cel generatie kits
  • Complete ES/iPS cel Pluripotente Analyse (van Biomarker Kits tot In Vivo Teratoma Formatie Analyse)
  • Knock-out en transgene diensten
  • Knock-out en transgene diensten
  • Exclusieve TARGATTTM technologie: Snelle en kosteneffectieve plaatsspecifieke transgene muisdiensten
  • Exclusieve TARGATTTM-technologie: Genmodificatie in stamcellen
  • Exclusieve TARGATTTM technologie: Genereren van gehumaniseerde diermodellen
  • Exclusieve TARGATTTM technologie: Genereren van stabiele productie CHO-cellijnen voor hoge expressie van eiwitten





Website: www.appliedstemcell.com

 
Mycoplasma Detection Kits - Conventional PCR

Mycoplasma Detection Kits - Conventional PCR


Mycoplasma contamination is a serious problem for laboratories due to its impact on cell properties and safety of biological products. Mycoplasma contaminations in cell cultures are unnoticeable (macroscopically as well as microscopically), making routine tests for reliable mycoplasma detection necessary. They represent a serious concern for academic and biopharmaceutical laboratories due to their effects on cell properties and on safety of biological products.
Mycoplasma (term often interchangeably used with Mollicutes, which include Mycoplasma, Acholeplasma, and Spiroplasma) are ubiquitous in the environment. In cell culture laboratories, 95 % of all mycoplasma infections in continuous cell lines are caused by as few as six contaminant species of bovine, swine, and human origin. These microbial agents easily spread from an infected cell culture batch to another by aerosol transmission or inadequate aseptic techniques.

Regular mycoplasma testing and control procedures help avoiding the costly consequences of a cell culture contamination and ensuring safe biopharmaceuticals and reliable scientific results. Consequently, most scientific publishers do not accept submission of cell-based studies without proof of the performed mycoplasma tests.
Compared to alternative methods, PCR-based mycoplasma detection kits are rapid, simple and do not require viable mycoplasma, special equipment, or extensive staff training.

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