TransGen Biotech
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TransGen Biotech, Inc. is a researcher, developer, manufacturer and distributor of more than 200 molecular and cellular biology products and kits for life science research and molecular diagnostics. In 2001, the company was founded by three scientists with a mission to produce innovative and cost-effective products for life science research. Currently, our products cover: plasmid based DNA markers, high efficiency chemically competent cells, 5 minutes PCR product cloning and expression vectors, a variety of PCR enzymes and supermix, RNase H deficient and high temperature RT enzymes, qPCR and qRT-PCR supermix, the highest efficiency mutagenesis kits, high quality nucleic acid extraction and purification kits, unstained and prestained protein markers, western blot markers, and protein purification resins, cell culture and transfection reagents, antibodies.
Website : www.transgenbiotech.com
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![Affinity Resins](https://www.clinisciences.com/nl/upload/thumbs/affinity-ex2vkvvm-Fqs3nzAW-ff4635.jpeg)
Affinity Resins
Affinity chromatography relies on the specific and reversible binding of a protein to a ligand bound to the matrix, which is then referred to as an affinity resin. The ligand can either bind directly to the protein of interest or to a tag that is covalently attached to the protein (Histidine, GST ...). Affinity chromatography is often the most robust purification procedure and is generally used in the early stages of the purification process. Depending on the downstream application, affinity purification may be the only chromatographic step necessary to achieve adequate purity.
The proteins can be purified by affinity chromatography in a selective or non-selective manner. In selective affinity chromatography, a ligand specific for a covalently linked protein or tag is used. In non-selective affinity chromatography such as Protein A, G, L for immunoglobulins, or heparin for DNA binding proteins, or lectin for glycoproteins, the ligand binds to a group of proteins with Similar bonding capabilities.
Available resins :
- Nickel-NTA : for purification of poly-histidine tagged proteins
- Cobalt-NTA : for purification of poly-histidine tagged proteins
- GST : for purification of GST tagged proteins
- Protein A : for purification of antibodies
- Protein G : for purification of antibodies