TransGen Biotech
 |
||
TransGen Biotech, Inc. is a researcher, developer, manufacturer and distributor of more than 200 molecular and cellular biology products and kits for life science research and molecular diagnostics. In 2001, the company was founded by three scientists with a mission to produce innovative and cost-effective products for life science research. Currently, our products cover: plasmid based DNA markers, high efficiency chemically competent cells, 5 minutes PCR product cloning and expression vectors, a variety of PCR enzymes and supermix, RNase H deficient and high temperature RT enzymes, qPCR and qRT-PCR supermix, the highest efficiency mutagenesis kits, high quality nucleic acid extraction and purification kits, unstained and prestained protein markers, western blot markers, and protein purification resins, cell culture and transfection reagents, antibodies.
Website : www.transgenbiotech.com
| ||
qPCR mix
MasterMix is a ready-to-use reagent in which all the components are already mixed in an optimized way. Additional stabilizers are added to enable long-term storage.
The concentration of components is perfectly adapted to most of the assays.
Taq Polymerase
A HotStart Taq polymerase is inactive at low temperatures (room temperature). Heating at 95 °C activates the enzyme, and the amplification can begin once the primers are annealed. The enzyme is not active until the entire DNA is denatured.
dNTPs / dUTPs
Some kits contain a blend of dNTPs and dUTPs, other ones contain only dNTPs.
UNG
The Uracil-N-Glycosylase is an enzyme that hydrolyses all single-stranded and double-stranded DNA containing dUTPs.
ROX
Some thermocyclers require MasterMix containing ROX dye for normalization. This is the case for the ABI and Eppendorf machines, and optional on the Stratagene machines.
Fluorescein
For some thermocyclers, the normalization method for SYBR® Green assay uses Fluorescein to create a “virtual background”.
MgCl2
MgCl2 is necessary for the Reverse Transcriptase and the Taq activity. MgCl2 concentration in MasterMixes is optimized according to the amount of Taq and also the buffer composition.
Inert colored dye
Some buffers also include an inert colored dye, to enable visualization of the buffer when loading in the wells. This colored dye has no effect on the sensitivity of the assay and is a convenient working tool.
