TransGen Biotech
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TransGen Biotech, Inc. is a researcher, developer, manufacturer and distributor of more than 200 molecular and cellular biology products and kits for life science research and molecular diagnostics. In 2001, the company was founded by three scientists with a mission to produce innovative and cost-effective products for life science research. Currently, our products cover: plasmid based DNA markers, high efficiency chemically competent cells, 5 minutes PCR product cloning and expression vectors, a variety of PCR enzymes and supermix, RNase H deficient and high temperature RT enzymes, qPCR and qRT-PCR supermix, the highest efficiency mutagenesis kits, high quality nucleic acid extraction and purification kits, unstained and prestained protein markers, western blot markers, and protein purification resins, cell culture and transfection reagents, antibodies.
Website : www.transgenbiotech.com
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Reverse transcriptase PCR - RT-PCR
RT-PCR is a technique which makes it possible to make a PCR (polymerase chain reaction) from an RNA sample. RNA is first retrotranscribed by an enzyme called reverse transcriptase, which allows the synthesis of the complementary DNA (cDNA). The latter is then used to carry out a PCR. Reverse transcriptase or reverse transcriptase (RT) is an enzyme used by retroviruses and retrotransposons that transcribe the genetic information of viruses or retrotransposons from RNA to DNA, which can integrate into the genome of the host.
RT-PCR has been developed to use RNAs as a PCR amplification matrix. It is certainly the most sensitive method for detecting (and possibly quantifying) messenger RNAs at the level of an organ, tissue or cell. For this purpose, it is often carried out in situ (PCR in situ), ie on fixed biological material. It is also used for the construction of cDNA libraries, sorting of mRNA (Differencial Display RT-PCR) as well as the construction of DNA probes.
