TransGen Biotech
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TransGen Biotech, Inc. is a researcher, developer, manufacturer and distributor of more than 200 molecular and cellular biology products and kits for life science research and molecular diagnostics. In 2001, the company was founded by three scientists with a mission to produce innovative and cost-effective products for life science research. Currently, our products cover: plasmid based DNA markers, high efficiency chemically competent cells, 5 minutes PCR product cloning and expression vectors, a variety of PCR enzymes and supermix, RNase H deficient and high temperature RT enzymes, qPCR and qRT-PCR supermix, the highest efficiency mutagenesis kits, high quality nucleic acid extraction and purification kits, unstained and prestained protein markers, western blot markers, and protein purification resins, cell culture and transfection reagents, antibodies.
Website : www.transgenbiotech.com
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Mycoplasma Detection Kits - Conventional PCR
Mycoplasma contamination is a serious problem for laboratories due to its impact on cell properties and safety of biological products. Mycoplasma contaminations in cell cultures are unnoticeable (macroscopically as well as microscopically), making routine tests for reliable mycoplasma detection necessary. They represent a serious concern for academic and biopharmaceutical laboratories due to their effects on cell properties and on safety of biological products.
Mycoplasma (term often interchangeably used with Mollicutes, which include Mycoplasma, Acholeplasma, and Spiroplasma) are ubiquitous in the environment. In cell culture laboratories, 95 % of all mycoplasma infections in continuous cell lines are caused by as few as six contaminant species of bovine, swine, and human origin. These microbial agents easily spread from an infected cell culture batch to another by aerosol transmission or inadequate aseptic techniques.
Regular mycoplasma testing and control procedures help avoiding the costly consequences of a cell culture contamination and ensuring safe biopharmaceuticals and reliable scientific results. Consequently, most scientific publishers do not accept submission of cell-based studies without proof of the performed mycoplasma tests.
Compared to alternative methods, PCR-based mycoplasma detection kits are rapid, simple and do not require viable mycoplasma, special equipment, or extensive staff training.
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