Agarose Bead Technologies (ABT)
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Agarose Bead Technologies (ABT) houdt zich bezig met onderzoek en ontwikkeling van biotechnologische producten op basis van agarose. Het bedrijf biedt een breed scala aan niet-geactiveerde agarosekorrels voor Size Exclusion, evenals geactiveerde agarosekorrels voor Affiniteits- en ionenuitwisselingschromatografie (proteïne- en antilichaamzuiveringen) en vooraf geactiveerde harsen voor het koppelen van affiniteitsliganden.
ABT is een ISO 9001:2015 gecertificeerd bedrijf en vertrouwt op een sterk kwaliteitscontroleprogramma.
Website : www.abtbeads.es
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![Affinity Resins](https://www.clinisciences.com/nl/upload/thumbs/affinity-ex2vkvvm-Fqs3nzAW-ff4635.jpeg)
Affinity Resins
Affinity chromatography relies on the specific and reversible binding of a protein to a ligand bound to the matrix, which is then referred to as an affinity resin. The ligand can either bind directly to the protein of interest or to a tag that is covalently attached to the protein (Histidine, GST ...). Affinity chromatography is often the most robust purification procedure and is generally used in the early stages of the purification process. Depending on the downstream application, affinity purification may be the only chromatographic step necessary to achieve adequate purity.
The proteins can be purified by affinity chromatography in a selective or non-selective manner. In selective affinity chromatography, a ligand specific for a covalently linked protein or tag is used. In non-selective affinity chromatography such as Protein A, G, L for immunoglobulins, or heparin for DNA binding proteins, or lectin for glycoproteins, the ligand binds to a group of proteins with Similar bonding capabilities.
Available resins :
- Nickel-NTA : for purification of poly-histidine tagged proteins
- Cobalt-NTA : for purification of poly-histidine tagged proteins
- GST : for purification of GST tagged proteins
- Protein A : for purification of antibodies
- Protein G : for purification of antibodies