Biorbyt

Products for advancing your discoveries

 
From antibodies to proteins, biochemicals to ELISA kits, Biorbyt's high quality research products are available for you to enable world class research and contribution to a deeper understanding of life sciences.
 
A comprehensive catalog of life science research products covering the areas of Neuroscience, Signal transduction, Cell Biology, Cancer Metabolism, Cardiovascular and others
 
Product range :
 
  • Antibodies
Choose from over 100,000 primary antibodies and over 2,000 secondary antibodies. Monoclonals and polyclonals tested in a number of applications - WB, ELISA, IHC, FACS and many more.
 
  • Proteins
Choose from over 7,000 proteins and over 400 active proteins. All of our proteins have been validated for use in ELISAs, WB and IHC-P
 
  • Biochemicals
We have over 10,000 biochemicals for use in your research.
 
  • ELISA kits
We have over 2,000 ELISA kits covering many research areas . Each kit comes with a fully detailed protocol and examples of expected results.
 
 
Plant total RNA extraction - Precipitation

Plant total RNA extraction - Precipitation


The most common organic extraction is the method using phenol-chloroform solution
Phenol extraction is a commonly used method for removing proteins from a DNA sample, e.g. to remove proteins from cell lysate during genomic DNA preparation.
Organic extraction involves the addition of and incubation in multiple different chemical solutions; including a lysis step, a phenol chloroform extraction, an ethanol precipitation, and washing steps.
Samples are homogenized in a phenol-containing solution and then centrifuged after chloroform addition. During centrifugation, the sample separates into three phases: an upper aqueous phase which contains DNA, an interface containing the proteins, and a lower organic phase containing RNA and lipids.
The advantage of the phenol-chloroform method is the simplicity of the protocol, the rapid denaturation of nucleases and the stabilization of nucleic acids. The main disadvantage is the use of toxic chemicals phenol and chloroform, and there is an increased risk of contamination due to transferring the DNA between multiple tubes.

Resultaat van uw zoeken : 2 product gevonden

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RUOCE / IVD
  • Unconjugated 2
  • Buffers and reagents 1
  • kit 1
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orb640210-100ml
 100ml