Biorbyt
Products for advancing your discoveries From antibodies to proteins, biochemicals to ELISA kits, Biorbyt's high quality research products are available for you to enable world class research and contribution to a deeper understanding of life sciences.
A comprehensive catalog of life science research products covering the areas of Neuroscience, Signal transduction, Cell Biology, Cancer Metabolism, Cardiovascular and others
Product range :
Choose from over 100,000 primary antibodies and over 2,000 secondary antibodies. Monoclonals and polyclonals tested in a number of applications - WB, ELISA, IHC, FACS and many more.
Choose from over 7,000 proteins and over 400 active proteins. All of our proteins have been validated for use in ELISAs, WB and IHC-P
We have over 10,000 biochemicals for use in your research.
We have over 2,000 ELISA kits covering many research areas . Each kit comes with a fully detailed protocol and examples of expected results.
Website : https://www.biorbyt.com/
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F(ab')2 fragment secondary antibodies
Secondary antibodies can be classified based on their specificity, which can be broad or narrow, and their fragment type, which can be whole, F(ab), or F(ab')2.
The principle of F(ab')2 is to generate divalent antibody fragments that retain the antigen-binding Fab portions of whole IgG antibodies while removing most of the Fc region.
F(ab')2 fragments are generated by pepsin digestion of whole IgG antibodies to cleave the hinge region and leave intact some of the Fc region. The resulting fragments have two antigen-binding Fab portions linked together by disulfide bonds, making them divalent with a molecular weight of about 110 kDa. The use of F(ab')2 fragments has several advantages over whole IgG antibodies, including better penetration into tissues, reduced nonspecific binding to Fc receptors on live cells or to Protein A/G, and the ability to precipitate antigens. However, F(ab')2 fragments are not recommended for blocking since they have two binding sites that are available to capture the primary antibody introduced subsequently. The preparation of F(ab')2 fragments involves pepsin digestion of whole IgG antibodies, followed by purification using techniques such as FPLC with DEAE-
Resultaat van uw zoeken : 809 product gevonden
Verfijn uw zoekopdracht :
- Unconjugated 126
- FITC 124
- HRP 120
- Biotin 82
- AP 81
- TRITC 78
- Red 74
- RPE 32
- Cy3 14
- Cy5 12
- Cy5.5 12
- Cy7 12
- PE 11
- AMCA 10
- APC 9
- Glucose Oxidase 4
- Gold 2
- PE/Cy5 2
- PE/Cy5.5 2
- PE/Cy7 2
- RBITC 2
- human 214
- mouse 106
- rat 74
- rabbit 55
- goat 44
- guinea pig 40
- sheep 36
- horse 34
- cow 32
- cat 28
- dog 22
- pig 20
- hamster 18
- Secondary antibody
- rabbit 400
- goat 343
- donkey 48
- ELISA 456
- IHC 454
- WB 431
- IF 353
- FC 321
- FLISA 215
- Dot blot 65
- ChIP 2
- Polyclonal 727
- Goat 3