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Cell Data Sciences

De missie van het bedrijf is om het gebruik van geavanceerde moleculaire analysetechnieken op uitdagende biologische monsters mogelijk te maken en het herstel van kritieke genetische en genomische gegevens voor fundamentele wetenschap en gepersonaliseerde geneeskunde te verbeteren.


De RNAstorm-extractiekit, aangedreven door de gepatenteerde CAT5-technologie, verbetert de verwijdering van formaldehyde-geïnduceerde schade en levert RNA met een hogere opbrengst en kwaliteit, een betere integriteit en een grotere amplificeerbaarheid. De RNAstorm-kit is de beste oplossing voor RNA-seq, qPCR, microarray of andere genexpressieanalyses.

 
 
> Cell Data Sciences > Producten voor onderzoek > Moleculaire Biologie Gereedschap > Nucleic acids extraction
Nucleic acids extraction

Nucleic acids extraction


Nucleic acid extraction methods are essential to molecular biology and are commonly used in many medical and biological science applications.
Nucleic acid extraction can be divided into 3 steps, which can be optimized according to the type of sample and the downstream applications for which the nucleic acids will be used.  These steps are as follows:
1) Rupture of tissue and cell structures
2) Removal of proteins, lipids and other contaminants from nucleic acids
3) Transfer the nucleic acids to water or a buffer solution that will preserve them without interfering with subsequent work.

Nucleic acid extraction methods can be classified into two different types :
  • Solution methods (such as the phenol-chloroform method)
  • Methods based on the solid phase (such as magnetic beads or centrifugation columns).
Solid-phase extraction methods work by causing nucleic acids to bind to solid supports, such as magnetic beads coated with silica or other materials.  The beads (or other supports) are then washed with alcohol to remove contaminants.  The support is then washed with a liquid that makes the nucleic acids soluble again, which releases the DNA from the support in a process known as elution.  A simple method is to add a solution containing a chaotrope to the crude nucleic acid extract.  Chaotropic agents are molecules that disrupt the hydrogen bonding network between water molecules.  In the presence of chaotropes, nucleic acids are previously much less soluble and bind to glass, silica-covered magnetic beads and other solid supports - allowing them to be easily separated from contaminants.  Solid-phase extraction methods may also depend on selective binding of DNA and RNA to ion exchange resins or other chemicals.
RNA
RNA
DNA
DNA