β-Mercaptoethanol [60-24-2]
Referentie sc-202966A
Formaat : 100ml
Merk : Santa Cruz Biotechnology
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β-Mercaptoethanol (CAS 60-24-2)
ACCÈS RAPIDE AUX LIENS
Le bêta-mercaptoéthanol peut agir comme réactivateur enzymatique dans les systèmes nécessitant une réduction pour l'activation, et a été couramment utilisé pour réduire les liaisons disulfures afin de séparer les sous-unités protéiques pour les utiliser dans l'électrophorèse. Ce produit peut également être utilisé pour former de l'o-phtalaldéhyde-bêta-mercaptoéthanol (OPAME), un réactif fluorogène dont la réaction avec les amines primaires permet de détecter les acides aminés. Plus récemment, l'OPAME a été utilisé pour distinguer l'ADN ss de l'ADN ss et même les mésappariements d'une seule base dans l'hybridation de l'ADN.
β-Mercaptoethanol (CAS 60-24-2) Références:
- The activation mechanism of human porphobilinogen synthase by 2-mercaptoethanol: intrasubunit transfer of a reserve zinc ion and coordination with three cysteines in the active center. | Sawada, N., et al. 2005. J Biol Inorg Chem. 10: 199-207. PMID: 15747133
- Conformational change detection of DNA with the fluorogenic reagent of o-phthalaldehyde-beta-mercaptoethanol. | Long, YF., et al. 2008. J Phys Chem B. 112: 1783-8. PMID: 18215031
- Effect of thiol compounds on bleomycin-induced DNA and chromosome damage in human cells. | Mira, A., et al. 2013. Arch Environ Occup Health. 68: 107-16. PMID: 23428061
- Detection of plant oil DNA using high resolution melting (HRM) post PCR analysis: a tool for disclosure of olive oil adulteration. | Vietina, M., et al. 2013. Food Chem. 141: 3820-6. PMID: 23993554
- Cryo-EM Structures Reveal Mechanism and Inhibition of DNA Targeting by a CRISPR-Cas Surveillance Complex. | Guo, TW., et al. 2017. Cell. 171: 414-426.e12. PMID: 28985564
- Coordinated Control of mRNA and rRNA Processing Controls Embryonic Stem Cell Pluripotency and Differentiation. | Corsini, NS., et al. 2018. Cell Stem Cell. 22: 543-558.e12. PMID: 29625069
- A coculture system composed of THP-1 cells and 3D reconstructed human epidermis to assess activation of dendritic cells by sensitizing chemicals after topical exposure. | Schellenberger, MT., et al. 2019. Toxicol In Vitro. 57: 62-66. PMID: 30771472
- Stimulation of Hair Growth by Small Molecules that Activate Autophagy. | Chai, M., et al. 2019. Cell Rep. 27: 3413-3421.e3. PMID: 31216464
- Histone Modifications Regulate Chromatin Compartmentalization by Contributing to a Phase Separation Mechanism. | Wang, L., et al. 2019. Mol Cell. 76: 646-659.e6. PMID: 31543422
- SUMO-Binding Entities (SUBEs) as Tools for the Enrichment, Isolation, Identification, and Characterization of the SUMO Proteome in Liver Cancer. | Lopitz-Otsoa, F., et al. 2019. J Vis Exp.. PMID: 31736480
- N-glycan in the scavenger receptor cysteine-rich domain of hepsin promotes intracellular trafficking and cell surface expression. | Sun, S., et al. 2020. Int J Biol Macromol. 161: 818-827. PMID: 32553951
- Systematic Activity Maturation of a Single-Domain Antibody with Non-canonical Amino Acids through Chemical Mutagenesis. | Lindstedt, PR., et al. 2021. Cell Chem Biol. 28: 70-77.e5. PMID: 33217338
- Condensed Chromatin Behaves like a Solid on the Mesoscale InVitro and in Living Cells. | Strickfaden, H., et al. 2020. Cell. 183: 1772-1784.e13. PMID: 33326747
- SARS-CoV-2 Nsp14 activates NF- kappa B signaling and induces IL-8 upregulation. | Li, T., et al. 2021. bioRxiv.. PMID: 34075374
- Flow Cytometry-Based Determination of Ploidy from Dried Leaf Specimens in Genomically Complex Collections of the Tropical Forage Grass Urochloa s. l. | Tomaszewska, P., et al. 2021. Genes (Basel). 12: PMID: 34201593
- Jack bean urease (EC 3.5.1.5). III. The involvement of active-site nickel ion in inhibition by beta-mercaptoethanol, phosphoramidate, and fluoride. | Dixon, NE., et al. 1980. Can J Biochem. 58: 481-8. PMID: 7272836
État Physique :
Liquid
Solubilité :
Soluble in water (500 g/l), alcohol, ether, benzene, and other organic solvents.
Stockage :
Store at room temperature
Point de fusion :
<-100° C
Point d'Ébullition :
157.2° C (dec.)
Densité :
1.11 g/mL at 25° C (lit.)
Indice de Réfraction :
n20D 1.50 (lit.)
Valeurs pK :
pKa: 9.64 (Predicted)
WGK Allemagne :
3
RTECS :
KL5600000
PubChem CID :
1567Indice Merck :
14: 5869
Numéro MDL :
MFCD00004890
Numéro EC :
200-464-6
Registre Beilstein :
773648
SMILES :
OCCS
Télécharger la Fiche de Sécurité/ SDS (MSDS)
Région
EXEMPLE de Certificat d'Analyse (CoA)
β-Mercaptoethanol | CAS 60-24-2 SAMPLE Certificate of AnalysisCERTIFICAT D'ANALYSE
Numéro de catalogue
Numéro de Lot
SAMPLE Certificate of Analysis (COA)
| Test | Specification | Results |
|---|---|---|
| Appearance | Clear colorless liquid | Complies |
| Identification (1H-NMR) | Consistent with structure | Complies |
| Purity (GC) | ≥ 99.0% | 99.5% |
| Purity (Titration) | ≥ 98.5% (by Na2S2O3) | 101.5% |
| Solubility | Clear colorless at 1 ml/ml in water | Complies |
| Copper | ≤ 1.0 ppm | < 0.9 ppm |
| Iron | ≤ 1.0 ppm | < 0.9 ppm |
| Lead | ≤ 5.0 ppm | < 2.6 ppm |
| Zinc | ≤ 1.0 ppm | < 0.4 ppm |
| Cell Culture Test | Complies | |
| Suitable for electrophoresis | Complies |
Informations pour la commande
| Nom du produit | Ref. Catalogue | COND. | Prix HT | QTÉ | Favoris | |
β-Mercaptoethanol, 100 ml | sc-202966A | 100 ml | .00 | |||
β-Mercaptoethanol, 250 ml | sc-202966 | 250 ml | .00 |