Immunohistochemical staining of formalin fixed and paraffin embedded human tonsil tissue section using anti-human Ig lambda light chain rabbit monoclonal antibody, Clone RM127. ELISA showing RM127 reacts only to the lambda light chain of human immunoglobulins, and not to the kappa light chain, mouse IgG, rat IgG, or goat IgG. A titer ELISA using RM127. The plate was coated with different amounts of human IgG3λ. A serial dilution of RM127 was used as the primary antibody, followed by an alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody. ELISA showing RM127 does not react to monkey IgG. The plate was coated with Rhesus monkey IgG. A serial dilution of RM127 and a monkey IgG binding antibody (positive control) was used as the detection antibody.
Product Details Product Name Anti-Human Lambda Light Chain Rabbit Monoclonal Antibody, Clone RM127 Description Rabbit monoclonal to Human Lambda Light Chain Catalog No. 31-1029-00 Clone Name RM127 Product Data Sheet Anti-Human Lambda Light Chain Rabbit Monoclonal Antibody Data Sheet RM127 Specificity RM127 reacts to the lambda light chain of human immunoglobulins, and does not react to the kappa light chain. RM127 does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG. Immunogen Human IgG Application Immunohistochemistry (IHC)
Flow Cytometry (FC)
Immunocytochemistry (ICC)
ELISA Species Reactivity Human Conjugate None Intended Use Research Use Only . Properties Form Liquid Storage Instructions Store at -20.0°C Stability Stable for 1 Year at -20.0°C from date of receipt Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide Volume/Item Size 100 µg Concentration 1.0 mg/mL Purity Protein A affinity purified from an animal origin–free culture supernatant Clone Type Monoclonal Isotype Rabbit IgG Usage ELISA: 0.05 µg/mL – 0.2 µg/mL;
Immunocytochemistry (ICC): 0.5 µg/mL - 2 µg/mL;
Immunohistochemistry (IHC): 0.5 µg/mL - 2 µg/mL;
Flow Cytometry (FC): 0.2 µg/mL - 2 µg/mL. Keywords References for Human Ig Lambda Light Chain antibody [RM127] Cheung P et al. Single-Cell Chromatin Modification Profiling Reveals Increased Epigenetic Variations with Aging. Cell. (2018) 10.1016/j.cell.2018.03.079. Mass Cytometry; Human.Read more (PubMed: 29706550)
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Anti-Human Lambda Light Chain rabbit monoclonal antibody [RM127]
![Anti-Human Lambda Light Chain rabbit monoclonal antibody [RM127]](https://www.clinisciences.com/nl/upload/-img-lapin1.png)
Referentie 31-1029-00
Formaat : 100ug
Merk : RevMab Biosciences
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Rabbit monoclonal to Human Ig lambda light chain
100 µg
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