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Anti-LRRK2/Dardarin, N3 (Non-Mouse-Reactive) Antibody FL650 Conjugate

Afdrukken

Referentie 75-266-FL650

Formaat : 200uL

Neem contact op met een lokale distributeur :

Telefoonnummer : +1 850 650 7790

Merk : Antibodies Incorporated

Neem contact op met een lokale distributeur :

Telefoonnummer : +1 850 650 7790

Our Anti-Dardarin/LRRK2, N3 (non-mouse-reactive) mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N231B/34. It is KO validated, detects human, mouse, and rat Dardarin/LRRK2, N3 (non-mouse-reactive), and is purified by Protein A chromatography. It is great for use in IHC, ICC.

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC

Host Species

Mouse

Available Conjugates

Unconjugated

490

550

594

Data Sheet

KO Validated

SKU: 75-266-FL650

Product Details

Target

Dardarin/LRRK2, N3 (non-mouse-reactive)

Target Description

LRRK2 (also known as PARK8) encodes a protein with 5 putative functional domains: an N-terminal leucine-rich repeat (LRR) domain, a Roc (Ras of complex protein) domain that shares sequence homology to the Ras-related GTPase superfamily, a COR (C-terminal of Roc) domain, a mitogen-activated protein kinase kinase kinase (MAPKKK) domain, and a C-terminal WD40 repeat domain. Mutation in this gene is one of the most common causes of inherited Parkinson disease (Gandhi et al., 2008). LRRK2 was originally identified as a putative disease-causing transcript (DKFZp434H2111) within a 2.6-Mb region encompassing a locus for Parkinson disease-8 (PARK8). Northern blot analysis detected a 9-kb mRNA transcript in all tissues tested, including brain. The authors named the protein product dardarin, derived from the Basque word dardara, meaning tremor. LRRK2/dardarin is also known to positively regulate autophagy through a calcium-dependent activation of the CaMKK/AMPK signaling pathway and together with RAB29, plays a role in the retrograde trafficking pathway for recycling proteins, such as mannose 6 phosphate receptor (M6PR), between lysosomes and the Golgi apparatus in a retromer-dependent manner. LRRK2/PARK8 is also known to regulate neuronal process morphology in the intact central nervous system (CNS) and play a role in synaptic vesicle trafficking.

Format

Purified by Protein A chromatography

Concentration

0.5 mg/mL

Clonality

Monoclonal

Clone

N231B/34

Isotype

IgG2a

Applications

ICC, IHC

Host Species

Mouse

Gene Name

LRRK2 PARK8

Molecular Weight

>200 kDa

Antigen

Fusion protein amino acids 841-960 of human LRRK2 (accession number Q5S007) produced recombinantly in E. Coli

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2939959

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography and conjugation of purified mAb. Purified mAbs are >90% specific antibody.

Buffer

PBS with 0.09% azide

Conjugate

FL650 Ex: 655 nm, Em: 676 nm

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

12 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Leucine-rich repeat serine/threonine-protein kinase 2 (EC 2.7.11.1) (Dardarin)

UniProt Details

UniProt (Human): Q5S007
UniProt (Immunogen Species): Q5S007