OKEH02646 | APOE ELISA Kit (Human) Clinisciences

Referentie OKEH02646

Formaat : 96Wells

Neem contact op met een lokale distributeur :

Telefoonnummer : +1 850 650 7790

Merk : Aviva Systems Biology

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Neem contact op met een lokale distributeur :

Telefoonnummer : +1 850 650 7790

Datasheets/Manuals	Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.

Product Info

Predicted Species Reactivity

Homo sapiens, Human

Application

ELISA-Sandwich

ELISA Kit Detection Method

Colorimetric, OD450 nm

ELISA Kit Duration

~ 3 Hours

ELISA Kit Principle

Aviva Systems Biology APOE ELISA Kit (Human) (OKEH02646) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for APOE has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for APOE is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample APOE captured in well.

ELISA Kit Range

7.8-500ng/mL

ELISA Kit Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 91%

ELISA Kit Reproducibility

Mean Intra-assay CV%: < 4.1% (n = 20)
Mean Inter-assay CV%: < 7.8% (n = 20)

ELISA Kit Component

Component	Amount
Anti-APOE Microplate	96 Wells (12 x 8 Well strips)
APOE Lyophilized Standard	2
Sample Diluent	1 x 20 mL
100X Biotinylated APOE Detector Antibody	1 x 120 uL
100X Avidin-HRP Conjugate	1 x 120 uL
Detector Antibody Diluent	1 x 12 mL
Conjugate Diluent	1 x 12 mL
25X Wash Buffer	1 x 30 mL
TMB Substrate	1 x 10 mL
Stop Solution	1 x 10 mL

Reconstitution and Storage

Store as indicated in product manual.

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Sensitivity

3.1 ng/mL

Specificity

Natural and recombinant Human Apolipoprotein E

Assay Info

Assay Methodology: Quantitative Sandwich ELISA

Gene Symbol	APOE
Gene Full Name	apolipoprotein E
Alias Symbols	AD2;APO-E;ApoE4;apolipoprotein E;apolipoprotein E3;LDLCQ5;LPG.
NCBI Gene Id	348
Protein Name	P02649
Description of Target	Apolipoprotein E
Uniprot ID	https://www.uniprot.org/uniprot/P02649
Protein Accession #	NP_000032.1
Nucleotide Accession #	NM_000041.3
Protein Size (# AA)	317
Molecular Weight	36 kDa

Biological pathways

Name	# of Products
Anti-apoptosis	727
Cdc42 protein signal transduction	53
Cell death	254
CGMP-mediated signaling	27
Cholesterol efflux	92
Cholesterol homeostasis	162
Chylomicron remnant clearance	32
Cytoskeleton organization	135
G-protein coupled receptor signaling pathway	315
High-density lipoprotein particle assembly	58
High-density lipoprotein particle clearance	64
High-density lipoprotein particle remodeling	84
Induction of apoptosis	395
Intracellular transport	37
Negative regulation of blood vessel endothelial cell migration	49
Negative regulation of cholesterol biosynthetic process	26
Negative regulation of endothelial cell proliferation	73
Negative regulation of inflammatory response	84
Negative regulation of MAP kinase activity	109
Negative regulation of platelet activation	62
Nitric oxide mediated signal transduction	69
Phospholipid efflux	83
Positive regulation of cGMP biosynthetic process	17
Positive regulation of cholesterol efflux	69
Positive regulation of cholesterol esterification	75
Positive regulation of low-density lipoprotein particle receptor catabolic process	17
Positive regulation of membrane protein ectodomain proteolysis	87
Positive regulation of nitric-oxide synthase activity	101
Receptor-mediated endocytosis	92
Regulation of axon extension	18
Regulation of neuronal synaptic plasticity	24
Response to reactive oxygen species	25
Reverse cholesterol transport	103
Synaptic transmission, cholinergic	52
Triglyceride metabolic process	52
Very-low-density lipoprotein particle clearance	19
Very-low-density lipoprotein particle remodeling	37

Diseases

Name	# of Products
Alzheimer disease	138
Amyloidosis	223
Disease mutation	5332
Hyperlipidemia	41
Neurodegeneration	458

APOE Antibody - N-terminal region (ARP54283_P050)
Catalog #: ARP54283_P050

Species Tested: Human, Mouse, Rat

Application: WB, IHC

Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
APOE Antibody (OAEB02438)
Catalog #: OAEB02438

Application: P-WB

Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Size: 100UG
Sample Type: 2. mouse brain extracts (80ug)
3. rat brain extract (80ug)
Primary Antibody Dilution: 2ug/ml
Secondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR Bioscience
Secondary Antibody Dilution: 1: 20,000
Image Submitted by: Yuzhi Chen
University of Arkansas for Medical Science " data-size="300x300" class="product-image-wrapper cover">

APOE Antibody - N-terminal region (ARP54284_P050)
Catalog #: ARP54284_P050

Species Tested: Human, Mouse

Application: IHC, WB

Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
A. 30 ug HepG2 whole cell lysate/extract
12 % SDS-PAGE
Apolipoprotein E antibody [C2C3], C-term (OAGA01162) dilution: 1:1000" data-size="300x300" class="product-image-wrapper cover">

APOE Antibody - C-terminal region (OAGA01162)
Catalog #: OAGA01162

Application: ELISA, FA, WB

Format: Liquid 1XPBS, 1%BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Size: 100 ul
APOE ELISA Kit (Mouse) : 96 Wells (OKCA00134)
Catalog #: OKCA00134

Kit Range: 6.25 ng/mL - 400 ng/mL

Sensitivity: < 1.56 ng/mL

Size: 96 Wells

APOE ELISA Kit (Human)

Referentie OKEH02646

Neem contact op met een lokale distributeur :

Merk : Aviva Systems Biology

Neem contact op met een lokale distributeur :

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