OKBB00685 | BMP2 ELISA Kit (Horse) Clinisciences

Datasheets/Manuals	Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.

Product Info

Predicted Species Reactivity

Horse

Application

ELISA

ELISA Kit Detection Method

Colorimetric, OD450 nm

ELISA Kit Duration

~ 3 Hours

ELISA Kit Principle

Aviva's Equine BMP-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for BMP-2 has been precoated onto 96-well plates. Standards(CHO, Q283-R396) and test samples are added to the wells, a biotinylated detection monoclonal antibody from mouse specific for BMP-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the Equine BMP-2 amount of sample captured in plate.

ELISA Kit Range

31.2 pg/ml - 2,000 pg/ml

ELISA Kit Reproducibility

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	285	742	1197	306	834	1358
Standard deviation	9.12	23.17	37.12	18.97	58.38	97.78
CV(%)	3.2	3.5	3.1	6.2	7	7.2

ELISA Kit Component

Component	Amount
96-Well Plate Pre-coated with Anti-Horse BMP2 Antibody	96 Wells (12 x 8 Well Strips)
Lyophilized Recombinant Horse BMP2 Standard	2 x 10 ng
100X Biotinylated Anti-Horse BMP2 Antibody	1 x 130 uL
100X Avidin-Biotin Peroxidase Complex (ABC)	1 x 130 uL
Sample Diluent Buffer	1 x 30 mL
Antibody Diluent Buffer	1 x 12 mL
ABC Diluent Buffer	1 x 12 mL
TMB Color Developing Agent	1 x 10 mL
TMB Stop Solution	1 x 10 mL
10X Wash Buffer	1 x 30 mL

Additional Information

To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

Lots	Lot1 (pg/ml)	Lot2 (pg/ml)	Lot3 (pg/ml)	Lot4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	55	51	58	51	53	2.94	5.5%
Sample 2	250	245	240	275	252	13.46	5.3%
Sample 3	1005	885	885	952	931	50.36	5.4%

*number of samples for each test n=16.

::

Standard Number	8	7	6	5	4	3	2	1
Concentration (pg/ml)	0	2000	2000	2000	2000	2000	2000	2000
O.D.	0.005	0.034	0.059	0.119	0.277	0.680	1.642	2.417

Reconstitution and Storage

Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Sample Type

Sandwich High Sensitivity ELISA kit for Quantitative Detection of horse equine BMP-2

Sensitivity

<2 pg/ml

Immunogen

Expression system for standard: CHO; Immunogen sequence: Q283-R396

Gene Symbol	BMP2
Gene Full Name	bone morphogenetic protein 2
Alias Symbols	Bone morphogenetic protein 2 ;BMP2 ;
NCBI Gene Id	100051701
Protein Name	Bone morphogenetic protein 2
Description of Target	Bone morphogenetic protein-2(BMP-2, BMP 2A) belongs to the transforming growth factor beta(TGF-beta) superfamily. It is thought to be involved in cartilage and bone formation during embryogenesis, but may have additional functions in morphogenesis as implied by its expression in various organs and embryonic tissues of mice.1 BMP-2 has been identified as a candidate mediator of retinoid activity. BMP-2 protein induces medulloblastoma cell apoptosis, whereas the BMP-2 antagonist noggin blocks both retinoid and BMP-2-induced apoptosis. BMP-2 also induces p38 mitogen-activated protein kinase(MAPK), which is necessary for BMP-2 and retinoid-induced apoptosis.2 Bone morphogenic proteins(BMPs) are known to promote osteogenesis, and clinical trials are currently underway to evaluate the ability of certain BMPs to promote fracture-healing and spinal fusion.3 The standard product used in this kit is recombinant horse equine BMP-2, constituting dimer by two chains of 114 amino acids with the molecular mass of 26KDa.
Uniprot ID	C8C060
Protein Accession #	NP_001191.1
Nucleotide Accession #	NM_001200.3

BMP2 ELISA Kit (Horse)

Referentie OKBB00685

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