BOVINE SERUM ALBUMIN - Fraction V (Immunoglobulin and Protease Free)
Referentie OORA01663
Formaat : 10g
Merk : Aviva Systems Biology
Datasheets/Manuals | Printable datasheet for OORA01663 |
---|
Product Format | Lyophilized |
---|---|
Conjugation | Unconjugated |
Application | ELISA, FA, WB |
Reconstitution and Storage | 2°C to 8°C |
Purity | 100% by Agarose Zone electrophoresis |
Specificity | Moisture (%) 0.5 % Loss on Drying pH: 7.0 Ash: 1.0% Protein (%): 98.9 % by nitrogen analysis. Protease: <0.005 Units/mg Heavy Metals: (Pb) < 0.50 ppm by ICP Endotoxin: < 0.1 EU/mg by LAL |
Application Info | This product is suitable for use in protease sensitive assays such as RIA, EIA and nucleic acid hybridization, use as a stabilizing agent for proteins and enzymes including dilute solutions of antibody, use as a blocking agent to reduce non-specific binding. ELISA : Yes FLISA : Yes IF Microscopy : Yes Western Blot : Yes Immunohistochemistry: Yes Flow cytometry: Yes |
Alias Symbols | BSA Blocker, BSA Blocking |
---|---|
Description of Target | Bovine Serum Albumin (BSA) is used for various biochemical applications including ELISA (Enzyme-Linked Immunosorbent Assay), high content screenining assays, western blotting, FACS Buffer and immunohistochemistry. BSA as a blocking reagent is particularly useful with casein-sensitive antibodies, such as phospho-specific antibodies. Also used as a nutrient in cell and microbial culture. In restriction digests, BSA is used to stabilize some enzymes during digestion of DNA and to prevent adhesion of the enzyme to reaction tubes and other vessels. Bovine Serum Albumin can also be used to determine the quantity of other proteins, by comparing an unknown quantity of protein to known amounts of BSA. |