CCL5 ELISA Kit (Dog)
Referentie OKEH07306
Formaat : 96T
Merk : Aviva Systems Biology
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Telefoonnummer : +1 850 650 7790
| Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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| Predicted Species Reactivity | Canis lupus, Dog | ||||||||||||||||||||||
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| Application | ELISA-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology CCL5 ELISA Kit (Dog) (OKEH07306) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CCL5 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CCL5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CCL5 captured in well. | ||||||||||||||||||||||
| ELISA Kit Range | 39-2500pg/mL | ||||||||||||||||||||||
| ELISA Kit Recovery | Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 84% (n = 20) | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Mean Intra-assay CV%: <=4.3% (n = 20) Mean Inter-assay CV%: <=5.5% (n = 20) | ||||||||||||||||||||||
| ELISA Kit Component |
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| Additional Information | Function: Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. | ||||||||||||||||||||||
| :: | Subcellular Location: Secreted | ||||||||||||||||||||||
| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sensitivity | 19.5pg/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant Dog C-C motif chemokine 5 | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
| Gene Symbol | CCL5 |
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| Gene Full Name | C-C motif chemokine ligand 5 |
| Alias Symbols | C-C motif chemokine 5;RANTES;small-inducible cytokine A5;T-cell-specific protein RANTES. |
| NCBI Gene Id | 403522 |
| Protein Name | Q8HYS0 |
| Description of Target | C-C motif chemokine 5 |
| Uniprot ID | https://www.uniprot.org/uniprot/Q8HYS0 |
| Protein Accession # | NP_001003010.1 |
| Nucleotide Accession # | NM_001003010.2 |