CD4 (CD4 Receptor, CD4mut, T Cell Surface Antigen T4/Leu3, T Cell Surface Glycoprotein CD4) (APC)

Referentie C2255-27B-APC-100ul

Formaat : 100ul

Merk : US Biological

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C2255-27B-APC CD4 (CD4 Receptor, CD4mut, T Cell Surface Antigen T4/Leu3, T Cell Surface Glycoprotein CD4) (APC)

Clone Type
Polyclonal
Host
mouse
Source
rat
Swiss Prot
P05540
Isotype
IgG2a
Grade
Affinity Purified
Applications
IHC
Crossreactivity
Rt
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze

CD4, a single chain transmembrane glycoprotein is found on a T cell subset (helper/inducer) representing 45% of peripheral blood lympocytes. It is also present on 80% of thymocytes and at a lower level on monocytes. It is involved in recognition of antigen presented along with MHC class II by APCs. It serves as receptor for HIV. Antibody to CD4 recognizes T-helper cells required for recognition of class II antigens. It reacts with 60% of peripheral blood E rosette-positive (E+) cells while showing negligible reactivity with E+ cells, monocytes, granulocytes, EBV-transformed B cell lines, and mouse splenocytes.|CD4-positive T-cells provide helper funciton in pokeweed mitogen driven B-cell differentiation. Moreover, anti-CD4 selectively blocks the autologus mixed lymphocyte reaction (AMLR) with minimal or no effect on the allogeneic MLR (allo-MLR). Blocking of the autoreactivity occurs when either autologus B-lymphocytes or macrophages are used as stimulators. Specificity closely approximates the binding spectrum of OKT4 and Leu-3 antibodies both of which also recognize 55 to 65% of human peripheral blood T-cells.||Applications:|Suitable for use in Immunohistochemistry. Other applications have not been tested.||Recommended Dilution:|Immunohistochemistry: Frozen|Optimal working dilutions to be determined by researcher.||Recommended Secondary Reagents: |Goat anti-mouse IgG: HRP conjugate (non-cross reacting with rat IgG), IgG Goat anti mouse IgG: RPE (rat adsorbed)||Hybridoma: |Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS1 myeloma cell line||Recommended Negative Controls: |Mouse IgG2a Negative Control||Storage and Stability: |May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.||Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG2a|Clone No: OX-35|Host: mouse|Source: rat|Concentration: As Reported|Form: Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).|Purity: Purified by Protein A affinity chromatography.|Immunogen: MLR generated rat T helper lymphocytes.|Specificity: Recognizes the rat CD4 cell surface antigen, a 55kD glycoprotein expressed by helper T cells and weakly by monocytes. ||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
MLR generated rat T helper lymphocytes.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes the rat CD4 cell surface antigen, a 55kD glycoprotein expressed by helper T cells and weakly by monocytes.
References
1. Wang, C. et al. (1996). Immunohistochemical study of amoeboid microglial cells in fetal rat brain. J. Anat. 189: 567-574.