Assay Principle:

The DetectX® Urinary Creatinine Detection Kit offers a precise and efficient method to measure creatinine levels in urine samples. Designed for simplicity and speed, the kit enables completion of the assay in just 30 minutes. For accurate execution, consult the detailed instructions in the kit insert. Use the included creatinine standard to produce a reliable standard curve essential for the assay.

Protocol Summary:

• Introduce standards or diluted samples into the clear microtiter plate.
• Add DetectX® Creatinine Reagent to each well, ensuring thorough mixing.
• Incubate the mixture at room temperature for 30 minutes to facilitate the color-generating reaction.
• Employ a plate reader to measure the generated signal at 490nm and determine creatinine concentration using the standard curve.

Background:

Creatinine, scientifically known as 2-amino-1-methyl-5H-imidazole-4-one, plays a critical role in understanding renal function and muscle metabolism. As a breakdown product of phosphocreatine, a key molecule in energy storage within tissues, creatinine levels provide insights into the body’s metabolic state. The body synthesizes creatinine from dietary creatine and the amino acids arginine, glycine, and methionine, primarily in the kidneys and liver.

The conversion of creatine to creatinine, a non-enzymatic process, signifies the balance between muscle mass and renal excretion. As this conversion rate remains relatively constant under normal conditions, creatinine serves as a reliable indicator for normalizing urinary biomarkers. This stability makes it particularly valuable in medical diagnostics and research, especially in assessing renal function and muscle disorders.

Moreover, alterations in creatinine levels can signal various health conditions, including kidney disease, muscle wasting, and other metabolic disorders. Its consistent production rate and easy detection in urine make it a key parameter in clinical diagnostics and research.

The DetectX® Urinary Creatinine Detection Kit provides a straightforward, accurate method for measuring urinary creatinine in biomedical research, aiding in the understanding of renal health, muscle metabolism, and the impact of various diseases on these systems.