Cytokeratin 14 (HRP)

Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight from between 40kD and 68kD and isoelectric pH between 4.9 – 7.8, The individual cytokeratin polypeptides are designated 1–20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies||Applications: |Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.||Recommended Dilution:|Immunohistochemistry: Frozen sections|Optimal dilutions to be determined by the researcher.||Storage and Stability:|Store product at 4°C. For long-term storage, aliquot and store at 4°C. Aliquots are stable at 4°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. ||Note: Applications are based on unconjugated antibody.