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> F(ab')2 Rat IgM (mu chain) antibody (RPE)

F(ab')2 Rat IgM (mu chain) antibody (RPE)

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Referentie orb348349-1ml

Formaat : 1ml

Merk : Biorbyt

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    F(ab')2 Rat IgM (mu chain) antibody (RPE)

    F(ab')2 Rat IgM (mu chain) antibody (RPE)

    Catalog Number: orb348349

    Catalog Numberorb348349
    CategoryAntibodies
    DescriptionF(ab')2 Rat IgM (mu chain) antibody (RPE)
    Species/HostGoat
    ClonalityPolyclonal
    Tested applicationsFC, IF
    ReactivityRat
    IsotypeIgG F(ab')2
    ImmunogenRat IgM whole molecule
    Concentration0.5 mg/mL
    Dilution rangeFC: 1:100 - 1:250, IF: 1:100 - 1:250
    Form/AppearanceLyophilized
    PurityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Goat Serum, Rat IgM, and Rat Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). No reaction was observed against other rat heavy or light chain proteins.
    ConjugationRPE
    StorageStore vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.
    Buffer/Preservatives0.01% (w/v) Sodium Azide
    Alternative namesGoat F(ab')2 Anti-Rat IgM (mu chain) Phycoerythrin
    NoteFor research use only
    Application notesF(ab')2 Anti-Rat IgM Antibody Phycoerythrin conjugation is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.
    Expiration Date12 months from date of receipt.