Fab Anti-RABBIT IgG (H&L) (GOAT) Antibody

Referentie 811-1102

Formaat : 1mg

Merk : Rockland Immunochemicals

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Telefoonnummer : +1 850 650 7790

Specifications for Fab Rabbit IgG (H&L) Antibody

Product Details

Goat Fab Anti-Rabbit IgG (H&L) Antibody - 811-1102
Goat Fab Anti-Rabbit IgG Antibody, Goat Fab Fragment Anti-Rabbit IgG Antibody
Goat
IgG (H&L)
Polyclonal
IgG Fab

Target Details

Rabbit
Rabbit IgG whole molecule
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum.  No reaction was observed against anti-Papain or anti-Goat IgG F(c).

Application Details

Dot Blot
IF  - View References
Fab Anti-Rabbit IgG (H&L) Antibody has been tested by dot blot and is suitable for highly specific immunological methods requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

Formulation

1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None

Shipping & Handling

Wet Ice
Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.

Background

Fab Anti-Rabbit IgG (H&L) Antibody generated in goat detects immunoglobulin g from rabbit, both heavy and light chains of the antibody molecule are present. Each IgG has two antigen binding sites. Representing approximately 75% of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

References (3)

(). Ciliary Proteins Repurposed by the Synaptic Ribbon: Trafficking Myristoylated Proteins at Rod Photoreceptor Synapses. International Journal of Molecular Sciences
Applications
IF, Confocal Microscopy
PubMed
(). Endothelin receptor antagonists alleviate blood-brain barrier disruption and cerebral edema in a mouse model of traumatic brain injury: A comparison between bosentan and ambrisentan. Neuropharmacology
Applications
Undefined
PubMed
(). Early auto-immune targeting of photoreceptor ribbon synapses in mouse models of multiple sclerosis. EMBO Molecular Medicine
Applications
IF, Confocal Microscopy
PubMed

Related Protocols

Adherent Cell Lysis Protocol
General Immunoblotting Protocol
Immunocytochemistry (ICC) Protocol
Immunofluorescence (IF) Protocol
Suspension Cultured Cell Lysis Protocol

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Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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