| Component | FM111-01 (10 rxns) | FM111-02 (20 rxns) |
| 2×TransStart® FastPfu Fly PCR SuperMix | 250 μl | 500 μl |
| DMT Enzyme (10 units/μl) | 10 μl | 20 μl |
| DMT Chemically Competent Cell | 10×50 μl | 20×50 μl |
| Nuclease-free Water | 1 ml | 1 ml |
| SControl Plasmid (5 ng/μl) | 10 μl | 20 μl |
| SControl Primers (10 μM ) | 10 μl | 20 μl |
Fast Mutagenesis System
Catalog Number： FM111-01
Price：Please inquire first
Specification：
10 rxns
20 rxns
Product Details
• Primers anneal to the DNA template, mutant strands are synthesized with 2×TransStart® FastPfu PCR SuperMix.
• In vitro digestion of non-mutated parental plasmid (methylated plasmid) with DMT enzyme and in vivo degradation of non-mutated parental plasmid (methylated plasmid) with DMT Chemically Competent Cell, so as to efficiently select mutant clones.
Features
• Mutation sites on both primers to improve mutation efficiency.
• Partially overlapping primers for exponential DNA amplification.
• Fast (4 kb/min) and high fidelity ( 54-fold fidelity as compared to EasyTaq® DNA Polymerase) 2xTransStart® FastPfu PCR SuperMix for DNA amplification.
• Double digestions (in vitro and in vivo) of parental plasmids to enhance mutation efficiency.
Storage
Competent Cell at -70°C for six months; others at -20°C for two years
Shipping
Dry ice (-70℃)
Product Composition
References
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2 Ke Y, Lv X, Fu X, et al. Poly (ADP-ribosyl) ation enhances HuR oligomerization and contributes to pro-inflammatory gene mRNA stabilization[J]. Cellular and Molecular Life Sciences, 2021.(IF 6.49)
3 Ouyang M, Li X, Zhang J, et al. Liquid-liquid phase transition drives intra-chloroplast cargo sorting[J]. Cell, 2020.(IF 66.85)
4 Song N, Qi Q, Cao R, et al. MAVS O-GlcNAcylation is essential for host antiviral immunity against lethal RNA viruses[J]. Cell reports, 2019.(IF 7.81)
5 Hu Z, He J, Chen K, et al. Molecular cloning and biochemical characterization of a new flavonoid glycosyltransferase from the aquatic plant lotus[J]. Biochemical and biophysical research communications, 2019.(IF 12.10)
6 You L, Shi J, Shen L, et al. Structural basis for transcription antitermination at bacterial intrinsic terminator[J]. Nature communications, 2019.(IF 11.80)
7 Liu M, Xu B, Ma Y, et al. Reversible covalent inhibitors suppress enterovirus 71 infection by targeting the 3C protease[J]. Antiviral Research, 2021.(IF 5.97)
8 Liu X, Liu Z, Wu Z, et al. Resurrection of endogenous retroviruses during aging reinforces senescence[J]. Cell, 2023.(IF 66.85)
9 Tian Y, Chen Z H, Wu P, et al. MIR497HG‐Derived miR‐195 and miR‐497 Mediate Tamoxifen Resistance via PI3K/AKT Signaling in Breast Cancer[J]. Advanced Science, 2023.(IF 17.52)
10 Wang K, Zhang Z, Hang J, et al. Microbial-host-isozyme analyses reveal microbial DPP4 as a potential antidiabetic target[J]. Science, 2023.(IF 56.90)
11 Shi C, Yang X, Hou Y, et al. USP15 promotes cGAS activation through deubiquitylation and liquid condensation[J]. Nucleic Acids Research, 2022.(IF 14.90)
12 Chen Y G, Li D S, Ling Y, et al. A cryptic plant terpene cyclase producing unconventional 18‐and 14‐membered macrocyclic C25 and C20 terpenoids with immunosuppressive activity[J]. Angewandte Chemie, 2021.(IF 15.33)
13 Huang K, Wu X X, Fang C L, et al. Pol IV and RDR2: a two-RNA-polymerase machine that produces double-stranded RNA[J]. Science, 2021.(IF 63.71)
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