Reagentia en instrumenten voor immunologie, celbiologie en moleculaire biologie.
 
> FDG - Galactosidase Assay Kit

FDG - Galactosidase Assay Kit

Afdrukken Afdrukken
Log in alstublieft

Referentie CA083

Formaat : 500assays

Merk : Canvax Biotech

Neem contact op met een lokale distributeur :


Telefoonnummer : +1 850 650 7790

FDG – Galactosidase Assay Kit

Optimized, Stable & Convenient tool to quickly measure the levels of active β-galactosidase

FDG β-Galactosidase Assay Kit is an efficient, easy and highly sensitive tool to quickly measure the levels of active β-galactosidase expressed in cells transfected with plasmids expressing Lac Z.

Lac Z is often used reporter gene in experiments transfection because the β-galactosidase is very resistant to proteolytic degradation and its activity is easily measured. β-galactosidase performs the hydrolysis of fluorescein di-β-D galactopyranoside (FDG) into a fluorescein that can be detected at excitation/emission = 490/525 nm. The concentration of β-galactosidase is proportional to fluorescence produced.measure levels of active β-galactosidase expressed in cells transfected with plasmids expressing Lac Z.

500 assays
SKU: CA083 Categories: Cell Based Assays, Other Assays & Compounds

Detailed information:

  • Fast and convenient. 
  • Easy-to-use method to quantify the enzyme expression in transfected cells.
  • Sensitive: measure ß-galactosidase at femtogram level.

– 55 mL Reaction Buffer
– 25 mL Lysis Buffer
– 25 mL Stop solution
– 20 μL β-galactosidase enzyme (0.1 mg/mL)
– 500 μL β-Mercaptoethanol
– 125 μL FDG solution (10 mM)

Datasheet
MSDS
  • Measurement of β-Galactosidase activity in the lysates of transfected cell.

t

  • Strict quality controls in every phase of manufacturing to guarantee the highest quality and reproducibility.
  • Shipped in: Gel Pack.
  • Storage: Upon receipt, store the kit at -20 ºC. β-galactosidase substrate must be protected from light.

 

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

You may also like…

  • ONPG – Galactosidase Assay Kit

    CA080Add to basket

  • TBARS Assay Kit

    CA995Add to basket

  • XTT Cell Proliferation Assay Kit – 500 assays

    CA031-SAdd to basket

  • Resazurin Cell Viability Assay

    CA035-SAdd to basket