Specifications

Product Data
Applications	ID, IP
Recommended Dilution	Precipitating techniques as Immunoelectrophoresis and Single and Double Radial Immunodiffusion (Mancini, Ouchterlony) to identify and measure immunoglobulins; as secondary antibody to precipitate the immunoglobulin in normal Cat serum. Recommended Dilutions: Immunoelectrophoresis: Use 2 µl or equivalent against 120 µl antiserum. Double Radial Immunodiffusion: Use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells. Antibody Titre: Precipitin titre not less than 1/64 when tested against normal Cat serum in agar block titration.
Reactivities	Feline
Host	Goat
Clonality	Polyclonal
Immunogen	Purified normal IgG and fractions containing IgA and IgM and isolated from Cat serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity	The reactivity of the antiserum is directed to the major isotypes of the Cat immunoglobulin system as tested by immunoelectrophoresis and double radial immunodiffusion against pooled serum and purified immunoglobulins. Cross-reactivity Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
Formulation	State: Serum State: Lyophilized, Delipidated, Heat inactivated, Stable Whole Antiserum without preservatives
Form	Serum
Reconstitution Method	Restore by adding 1 ml of sterile distilled water.
Concentration	Total protein and IgG concentration in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added.
Conjugation	Unconjugated
Storage	Store lyophilized at 2-8°C and reconstituted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid Repeated thawing and freezing.
Note	Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other dog serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.