FSH ELISA Kit (Sheep)
Referentie OKCA00327
Formaat : 96Wells
Merk : Aviva Systems Biology
Neem contact op met een lokale distributeur :
Telefoonnummer : +1 850 650 7790
| Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
|---|
| Predicted Species Reactivity | Sheep | ||||||||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||||||||||||||||||||||
| ELISA Kit Duration | 2.5 Hours | ||||||||||||||||||||||||||||||||||||||||||
| ELISA Kit Linearity |
| ||||||||||||||||||||||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology FSH ELISA Kit (Sheep) (OKCA00327) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A Sheep monoclonal antibody specific for Sheep FSH has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells and incubated. After washing, a biotinylated detector antibody specific for Sheep FSH is added, incubated and followed by washing. Avidin-Biotin-Peroxidase Complex is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Sheep FSH captured in well. | ||||||||||||||||||||||||||||||||||||||||||
| ELISA Kit Range | 2 mI U/mL - 70mI U/mL | ||||||||||||||||||||||||||||||||||||||||||
| ELISA Kit Recovery |
| ||||||||||||||||||||||||||||||||||||||||||
| ELISA Kit Reproducibility |
| ||||||||||||||||||||||||||||||||||||||||||
| ELISA Kit Component |
| ||||||||||||||||||||||||||||||||||||||||||
| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||||||||||||||||||||||
| Sample Type | Serum, Plasma, Tissue homogenates | ||||||||||||||||||||||||||||||||||||||||||
| Sensitivity | 1 mIU/ml | ||||||||||||||||||||||||||||||||||||||||||
| Specificity | This assay has high sensitivity and excellent specificity for detection of sheep FSH. No significant cross-reactivity or interference between sheep FSH and analogues was observed. | ||||||||||||||||||||||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich Immunoassay | ||||||||||||||||||||||||||||||||||||||||||
| Gene Symbol | FSH |
|---|---|
| Gene Full Name | follicle stimulating hormone, beta polypeptide |
| NCBI Gene Id | 443387 |
| Protein Name | Follitropin subunit beta |
| Description of Target | The pituitary glycoprotein hormone family includes follicle-stimulating hormone, luteinizing hormone, chorionic gonadotropin, and thyroid-stimulating hormone. All of these glycoproteins consist of an identical alpha subunit and a hormone-specific beta subunit. This gene encodes the beta subunit of follicle-stimulating hormone. In conjunction with luteinizing hormone, follicle-stimulating hormone induces egg and sperm production. Alternative splicing results in two transcript variants encoding the same protein. |
| Uniprot ID | P01227 |
| Protein Accession # | NP_001009798.1 |
| Nucleotide Accession # | NM_001009798.1 |
| Protein Size (# AA) | 129 |
| Molecular Weight | 15 kDa |