Glucose (GLU) Fluorometric Assay Kit

Referentie E-BC-F037-48T

Formaat : 48T

Merk : Elabscience

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Detection Principle Glucose oxidase can catalyze the oxidation of glucose into gluconic acid and produce hydrogen peroxide. In the presence of peroxidase, hydrogen peroxide reacts with the non-fluorescent substance to form fluorescent substance. The glucose content can be calculated indirectly by measuring the fluorescence intensity at the excitation wavelength of 535 nm and the emission wavelength of 590 nm.
Synonyms GLU
Sample Type Serum,Plasma,Urine,Saliva,Milk,Cell
Detection Method Fluorometric method
Detection Instrument Fluorescence microplate reader (Ex/Em=535 nm/590 nm)
Research Area Metabolic Diseases ,  Glycolysis And Lipid Metabolism ,  Disulfidptosis ,  Energy Metabolism
Storage This product can be stored at -20°C for 12 months with shading light.
Valid Period 12 months
Sensitivity 0.1 μmol/L
Detection Range 0.1-20 μmol/L
Precision inter-assay CV: 2.8% | intra-assay CV: 1.7%
Assay Time 25 min
Cat.No. Product Name Sizes
E-BC-K234-M Glucose (Glu) Colorimetric Assay Kit (GOD-POD Method) 500Assays , 96T , 48T
E-BC-K234-S Glucose (Glu) Colorimetric Assay Kit (GOD-POD Method) 100Assays , 50Assays

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type Dilution Factor
Human plasma 300-600
Human serum 300-600
Chicken serum 600-1000
Human urine 1
Human milk 400-600
Saliva 3-5

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.

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