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> Glutathione Peroxidase (GSH-Px) Activity Assay Kit

Glutathione Peroxidase (GSH-Px) Activity Assay Kit

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Referentie E-BC-K096-S-50

Formaat : 50Assays

Merk : Elabscience

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Telefoonnummer : +1 850 650 7790

Detection Principle Glutathione peroxidase (GSH-Px) can promote the reaction of hydrogen peroxide (H2O2) and reduced glutathione to produce H2O and oxidized glutathione (GSSG). The activity of glutathione peroxidase can be expressed by the rate of enzymatic reaction. The activity of glutathione can be calculated by measuring the consumption of reduced glutathione. Hydrogen peroxide (H2O2) and reduced glutathione can react without catalysis of GSH-Px, so the portion of GSH reduction by non-enzymatic reaction should be subtracted. GSH can react with dinitrobenzoic acid to produce 5-thio-dinitrobenzoic acid anion, which showed a stable yellow color. Measure the absorbance at 412nm, and calculate the amount of GSH.
Detection Principle Image
Synonyms GSH-PX
Sample Type Serum,Plasma,Cell,Cell culture supernatant,Tissue
Detection Method Colorimetric method
Detection Instrument Spectrophotometer (412 nm)
Research Area Oxidative Stress ,  Plant Physiology Series ,  Ferroptosis
Other Reagents Required Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4)
Storage This product can be stored at 2-8°C for 12 months with shading light.
Valid Period 12 months
Sensitivity 12.65 U
Detection Range 12.65-387 U
Precision inter-assay CV: 9.3% | intra-assay CV: 4.9%
Assay Time 1 h 10 min
Cat.No. Product Name Sizes
E-BC-K030-S Reduced Glutathione (GSH) Colorimetric Assay Kit 100Assays , 50Assays
E-BC-K097-S Total Glutathione (T-GSH)/Oxidized Glutathione (GSSG) Colorimetric Assay Kit 100Assays , 50Assays
E-BC-K099-S Glutathione Reductase (GR) Activity Assay Kit 100Assays , 50Assays
E-BC-K278-S Glutathione-S-Transferase (GST) Activity Assay Kit 100Assays , 50Assays

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type Dilution Factor
Human serum 1-3
Mouse plasma 4-8
Rat serum 5-8
10% Mouse liver tissue homogenization 20-40

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.

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