Goat anti-Rabbit IgG (H&L), DyLight® 550 conjugated, min, cross-reactivity to bovine,goat,human, mouse,Rat IgG
Referentie AS111782
Formaat : 1mg
Merk : Agrisera
Goat anti-Rabbit IgG (H&L), DyLight® 550 conjugated, min, cross-reactivity to bovine,goat,human, mouse,Rat IgG
AS11 1782 | Clonality: Polyclonal | Host:Goat | Reactivity:Rabbit IgG (H&L)
-
This product doesn't have any reviews.
Host: | Goat |
Clonality: | Polyclonal |
Purity: | Immunogen affinity purified IgG. |
Format: | Lyophilized |
Quantity: | 1 mg |
Reconstitution: | For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily |
Storage: | Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming. |
Recommended dilution: | 1 : 50 -1 : 5000 (ICC), 1 : 20 -1 : 2000 (IHC), |
Application example ![]() Courtesy of Dr. Ferhan Ayaydin, Hungarian Academy of Science, Hungary Labeling and detection protocol Fixation (30 min) 4% paraformaldehyde in PBS (pH 7.4) with 0.01% TritonX-100. (3x5min PBS wash) Cell wall digestion (40 min): 1% Cellulase, 1% Pectinase in 0.5% (w/v) MES buffer (pH 5.6) (2x5 min PBS wash) Immobilization of cells (10mins): Cells in PBS were settled onto poly-L-Lysine coated coverslips, excess PBS removed without air drying the cells. Membrane permeabilization (10 min): 0.5% TritonX-100 in PBS (3x5min PBS wash) Blocking (10mins): 5% Fish gelatin in PBS Primary antibody incubation (2.5 h at 24°C): Agrisera (AS16 3690) rabbit anti-Cas9 polyclonal antibody diluted 1:100 in blocking buffer (4x5 min blocking buffer wash) Secondary antibody incubation (1h at 24°C): goat anti-rabbit DyLight 550 antibody (Agrisera AS11 1782) diluted 1:300 in blocking buffer. (3x5min PBS wash) Nuclear counterstaining (5 min): 200ng/ml DAPI in PBS (brief PBS wash) Mounting: Fluoromount G mounting medium was used to mount coverslips onto glass slides. Imaging: Olympus FV1000 confocal microscope with 40x (NA1.3) oil immersion objective. ![]() BiP localization in 4 days old Arabidopsis thaliana roots. BiP signal shown in red, Co-staining with DAPI visualized nucleus (blue color). The material has been fixed in para-formaldehyde for 30 minutes. Tissue cleaning has been performed before immunolocalization. Rabbit anti-BiP primary antibody diluted in 1: 400 and goat anti-rabbit IgG, pre-adsorbed secondary antibody DyLight®550 conjugated (Agrisera) have been used. Scale bar – 10 µm. Courtesy Dr. Taras Pasternak, Freiburg University |
Additional information: | This antibody reacts with the heavy (gamma) chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis. Minimum cross-reactivity is observed to non-immunoglobulin rabbit serum proteins and bovine,goat,human,mouse,rat IgG based on immunoelectrophoresis. Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.05 % (w/v) sodium azide as preservative. |
Background: | Goat anti-rabbit IgG (H&L), DyLight® 550 conjugated, is a secondary antibody which binds to rabbit IgGs in immunological assays. DyLight® 550 has Amax = 562 nm, Emax = 576 nm. Fluor to protein ratio is 2.47 moles DyLight® 550 per mole antibody. Antibodies are adsorbed against bovine,goat,human,mouse,rat IgG and are affinity purified using solid phase rabbit IgG. DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries. |